atac-see原理 ATAC-Seq(Assay for Transposase-Accessible Chromatin with high-throughput sequencing)即染色质可及性高通量测序技术,其原理主要基于转座酶可以特异性地结合在染色质开放区域这一特性,具体如下: 1. 染色质开放区域与转座酶的结合。 染色质的结构是动态变化的,在基因组中,有一些区域的染色质结构较为...
Integration of ATAC-see with flow cytometry enables automated quantitation and prospective cell isolation as a function of chromatin accessibility, and it reveals a cell-cycle dependence of chromatin accessibility that is especially dynamic in G1 phase. The integration of imaging and epigenomics provides...
Finally, ATAC-see can also be combined with fluorescence-activated cell sorting (FACS) to allow for cells to be sorted by their accessibility. This showed that accessibility was dependent on the stage of the cell cycle. Thus, ATAC-see also has the potential to uncover cellular heterogeneity. U...
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2016. ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing. Nature Methods 13:1013-1020. DOI: https://doi.org/10. 1038/nmeth.4031, PMID: 27749837Chen X, Shen Y, Draper W, Buenrostro JD, Litzenburger U, Cho SW, et al. ATAC-see reveals the accessible ...
Here we conducted a genome-wide CRISPR screening combined with an optimized ATAC-see protocol to identify genes that modulate global chromatin accessibility. In addition to known chromatin regulators like CREBBP and EP400, we discovered a number of previously unrecognized proteins that modulate chromatin...
ATAC-see labels accessible chromatin in the nucleus with fluorophores. We can image the labeled fluorescence distribution with single-molecule localization microscopy to obtain super-resolution images of the underlying structure of accessible chromatin in the nucleus. This project had many twists and ...