Furthermore, cell culture assays are needed that mimic the complexity of cell to cell communication at the same time as they provide control over the different microenvironmental parameters. Here, we aim to validate a previously developed microfluidic system for an astrocyte-neuron cell culture ...
Neural progenitor cells were differentiated in a neuron-astrocyte co-culture for 70 days, and dopamine-related gene and protein expression was investigated. Expression of genes specific for dopaminergic differentiation and functioning, such as LMX1B, NURR1, TH, SLC6A3, and KCNJ6, were...
Non-cell autonomous processes involving astrocytes have been shown to contribute to motor neuron degeneration in amyotrophic lateral sclerosis. Mutant superoxide dismutase 1 (SOD1) expression in astrocytes is selectively toxic to motor neurons in co-culture, even when mutant protein is expressed only in...
Co-culture Cell And Tissue Culture Decapitation Animal Euthanization FACS Cell Proliferation Assay ELISA Reagents Antibodies Protein Experimental Models Rattus norvegicus Kits Other Keywords Microglia Astrocytes Neuroinflammation References Streit WJ, Mrak RE, Griffin WS (2004) Microglia and neuroinflammation: a...
Astrocytes are a cell type critical to neuronal function, and the addition of astrocytes to neuron cultures can improve the quality of in vitro assays. Here, we present cellulose as an astrocyte culture substrate. Astrocytes cultured on the cellulose fiber matrix thrived and formed a dense 3D ...
NeuronAstrocyteCocultureNeuroprotectionGlutathioneHydrogen peroxideDopamineNeurodegenerationAstrocytes promote the survival of neurons. Conditions characterized by loss of neurons, such as aging and aging-related neurodegenerative disorders, are accompanied by both disturbances in astrocyte-neuron interactions and ...
In order to address whether transfer of PrPSc could occur from neurons to astrocytes, we set up co-culture experiments between the chronically prion-infected donor neuronal cell line ScCAD and naïve acceptor cerebel- lar astrocytes. ScCAD was chosen as a "neuron" donor instead of primary ...
Correction to: A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation 来自 Semantic Scholar 喜欢 0 阅读量: 9 作者:N Goshi,RK Morgan,PJ Lein,E Seker DOI: 10.1186/s12974-022-02391-4 年份: 2022 ...
Number of cells per cell culture condition n ≥ 22, from ≥2 differentiations. (d) Frequency of sPSC bursts per minute in co-cultures with primary human astrocytes + DAPT or neuron-only cultures equals the calcium oscillation frequency (Two-way ANOVA, p = NS for the ...
This co-culture study shows clearly the 'mutual induction' that astrocytes and endothelial cells exert on each other — free radical defence enzymes are upregulated in both cell types when they are grown together. Article CAS PubMed Google Scholar Verkman, A. S. Aquaporin water channels and ...