To distinguish them from the primary astrocytes, the bEND.3 cells (ATCC, No. CRL-2299) were incubated with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, 10 μM). The stained bEND.3 cells were cultured alone or with astrocytes at a 1:1 ratio in 12-well plates. Afte...
Cldn5 forward 5′- TAAGGCACGGGTAGCACTCA-3′, Cldn5 reverse 5′- GGACAACGATGTTGGCGAAC-3′, Acta2 forward 5′- GTCCCAGACATCAGGGAGTAA-3′, Acta2 reverse 5′- TCGGATACTTCAGCGTCAGGA-3′, RNA18s forward 5′- TTGAAAATCCGGGGGAGAG-3′; RNA 18s reverse 5′- ACATTGTTCCAACATGCCAG-3′...
HEK293T cell line ATCC CAT# CRL-3216; RRID: CVCL_0063 T11D7e2 Hybridoma ATCC CAT# TIB-103; RRID: CVCL_F769 Experimental Models: Organisms/Strains Rats: CD Sprague-Dawley Charles Rivers Stock #001; RRID: RGD_10395233 Experimental Models: Mice C57Bl6/J Jackson Laboratory Jax# 000664; RRID...
It has been shown that AAV-mediated gene delivery of glial cell line-derived neurotrophic factor into astrocytes exerted protective effects against dopaminergic neurotoxicity induced by MPTP and 6-OHDA (52). Because gene delivery of neurotrophic factors to neuronal cells can be distributed through long...
Clonal cell culture C6 rat glioma55and U87MG human astrocytoma56clonal cells were obtained from ATCC (Virginia, USA) and maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin)...
Cell culture and reagents HF66 cells were established at Henry Ford Midwest Neuro-Oncology Center, Detroit, MI [17]; A172, U251, LN18, C6, TNC-1 were purchased from the American Tissue Culture Collection (ATCC; Manassas, VA, USA). All the cells were cultured in Dulbeccos’ modified Eag...
there is evidence to support cell-autonomous or primary roles of astrocytes in HD. For example, human glial progenitor cell-derived astrocytes expressing mHTT can recapitulate motor aspects of the HD phenotype when implanted into mice, while control astrocytes can ameliorate HD pathology when implanted...
(ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101–0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento, CA, #100–106), nonessential amino acids (Hyclone, #SH30138.01), HEPES (Hyclone #30237.01), penicillin, streptomycin, and antifungal...
Freund’s adjuvant, complete (contains Mycobacterium tuberculosis (H37Ra, ATCC 25177), heat killed and dried) Sigma-Aldrich Cat# F5881 Freund’s adjuvant, incomplete (does not contain Mycobacteria tuberculosis) Sigma-Aldrich Cat# F5506 Pertussis Toxin List Biological Technologies Cat# 180 LFB Solven...
Neuro2A cells (mouse neuroblastroma cell line) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Neuro2A was cultured with DMEM containing 10% FBS and 1% penicillin-streptomycin at 37 °C in 5% CO2 incubator. Neuro2A was seeded in 24-well culture plates ...