STM4495- R TCACCGGTGGACATGCCTGC 0.5 1.4 基因组DNA的提取 根据细菌基因组DNA提取试剂盒说明书进行基因组DNA的提取。 1.5 多重PCR反应体系的建立及优化 首先以阳性菌株为模板进行单基因PCR扩增,建立单基因PCR反应体系。然后,采用方阵法优化引物浓度、退火温度,确定最佳PCR反应体系及反应条件,建立多重PCR方法。多重...
In light of this, we developed a Nanopore sequencing assay for HPV genotyping and compared its performance with cobas HPV Test and Roche Linear Array HPV Genotyping Test (LA). Methods: Two hundred and one cervicovaginal swabs were routinely tested for Papanicolaou smear, cobas HPV Test and LA....
The LAMP specific primers were selected for the detection of Sclerotinia sclerotiorum. The LAMP reaction system and optimal conditions were established, followed by sensitivity and specificity tests. The results demonstrated that F3-1: ATGCCTGTTCGAGCGTCA, B3-1: AGT...
When Viroselect was used to test a subset of inconclusive samples obtained from the COBAS 6800 platform, it was able to resolve approximately 94% of these samples. Most importantly, approximately 9% of these inconclusive results were found to be positive on Viroselect. This assay would thus ...
These methods have simpler test procedures and shorter time-consuming than conventional methods that were more convenient for rapid identification. Pulsatillae radix, Baitouweng (Chinese vernacular name), is well known as a commonly used traditional Chinese medicines (TCM) because of its notable ...
Results of the assay on 107 and 402 clinical samples from patients with suspected Lyme disease from Houston, Texas or received at the IGeneX laboratory in Palo Alto, California, respectively, were analyzed together with WB findings. The LM-PCR assay was highly specific for B. burgdorferi. In ...
Important test characteristics, such as the linearity, lower limit of detection (LLOD), lower limit of quantification (LLOQ), imprecision and inaccuracy were determined according to this report. 2.10.1. Linearity The calibration curve was generated using a 10 mg/L stock solution of quantitatively ...
accurate method to screen for the eight most-common disease-causing mutations, covering more than 98% of HBB alleles in the Tai- wanese population, using parallel minisequencing and multiplex assay by matrix-assisted laser desorption/ion- ization time-of-flight mass spectrometry (MALDI-TOF MS)....
The invention is directed to methods for determining the efficacy of treatment with telomerase modulators in mammals by the analysis of the level of telomerase reverse transcriptase
17.A method of determining the presence or amount of Botulinum toxin (BoNT) in a test sample, said method comprising the steps of:a) providing a support and one or more peptides for attachment thereto, wherein said one or more peptides have the formula “TAG-X-REP” in which:X is an...