A colorimetric coupled enzyme assay for the determination of 纬-glutamyltransferase (GGT) activity using glutathione as substrate is described. The cysteine released from glutathione upon sequential action of GGT and leucine aminopeptidase is spectrophotometrically detected through its reaction with ninhydrin ...
• GGT Assay Buffer • GGT Substrate • GGT Positive Control • pNA Standard (2mM) Description: The Gamma-GlutamylTransferase (GGT; EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and ...
Enzyme assay is usually carried out through the direct or indirect determination of the rate of digestion of the specific substrate, at different time intervals, after a given period of time. From: Agro-Industrial Wastes as Feedstock for Enzyme Production, 2016 ...
Metabolism Assay kits is mainly developed by using the principle of chemical reaction/enzymatic reaction or material structure characteristics to determine material content or enzyme activity in samples. CheKine™ Metabolism Assay kits independently de
Specific PCR-RFLP assay. PCR amplification was carried out with ITS2F and PR1 (5′- TGGGTCATCTTCT CCCAGC-3′in a final volume of 25 μL as described before. The PCR conditions were 1 cycle of 95 °C for 3 min, 35 cycles of 94 °C for 30 se, 62 °...
Naphthalimide-based fluorescent probe HDLGProbe12 for detection of gamma-glutamyltransferase(GGT) and imaging GGT in living cells Near-infrared tyrosinase responsive fluorescent probe HNLGNBR-AP for detection of tyrosinase (TYR) and used in Melanoma Imaging ...
Similar to SARS-CoV, the cellular receptor of SARS-CoV-2 is angiotensin-converting enzyme 2 (ACE2)3; however, the SARS-CoV-2 spike (S) protein has a 10- to 20-fold higher affinity for ACE2 than the corresponding S protein of SARS-CoV.4 The S prote...
Cell culture and indirect immunofluorescent detection have long been used for the detection of BEFV [17,[20], [21], [22]]. In addition, BEFV can be routinely detected by the conventional RT-PCR, real-time quantative(q) PCR, and enzyme-linked immunosorbent assay (ELISA) as well as the...
Vaidyanathan et al. Proteolysis of Snap-25 Isoforms by Botulinum Neurotoxin Types A, C, and E: Domains and Amino Acid Residues Controlling the Formation of Enzyme-Substrate Complexes and Cleavage; Journal of Neurochemistry, vol. 72, pp. 327-337. (Year: 1999). ...
As the 1-hydroxy-2-methyl-(E)-butenyl 4diphosphate reductase the IspH enzyme or a function-conservative variant thereof is preferably used. The gene of the IspH enzyme used in the assay of the invention may be derived from any organism having the non-mevalonate isoprenoid pathway, like plant...