• GGT Assay Buffer • GGT Substrate • GGT Positive Control • pNA Standard (2mM) Description: The Gamma-GlutamylTransferase (GGT; EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and ...
enzyme‐linked immunosorbent assayγ‐glutamyltransferasehepatocellular carcinomaAim: To develop a sandwich enzyme‐linked immunosorbent assay (ELISA) measuring hepatoma‐specific datura stramonium agglutinin–tightly bounding γ‐glutamyltransferase (DSA‐GGT) and evaluate its clinical application for hepatocellular ...
Enzyme assay is usually carried out through the direct or indirect determination of the rate of digestion of the specific substrate, at different time intervals, after a given period of time. From: Agro-Industrial Wastes as Feedstock for Enzyme Production, 2016 ...
(B) Restriction enzyme validation of the constructed bait vectors pGBKT7-(CMYA1/Xin repeat). 1: CMYA1 bait vector digested with EcoRI and SalI. 2: Xin repeat bait vector digested with EcoRI and BamHI. (C) Transformation of pGBKT7-Xin repeats into the Y2H Gold strain, autoactivation ...
Naphthalimide-based fluorescent probe HDLGProbe12 for detection of gamma-glutamyltransferase(GGT) and imaging GGT in living cellsNear-infrared tyrosinase responsive fluorescent probe HNLGNBR-AP for detection of tyrosinase (TYR) and used in Melanoma Imaging...
Metabolism Assay kits is mainly developed by using the principle of chemical reaction/enzymatic reaction or material structure characteristics to determine material content or enzyme activity in samples. CheKine™ Metabolism Assay kits independently de
Specific PCR-RFLP assay. PCR amplification was carried out with ITS2F and PR1 (5′- TGGGTCATCTTCT CCCAGC-3′in a final volume of 25 μL as described before. The PCR conditions were 1 cycle of 95 °C for 3 min, 35 cycles of 94 °C for 30 se, 62 °...
In addition, the quantitative range of the xylosylated peptides were validated, and the incubation time of the enzyme reaction was optimized for cell culture samples and human sera. The specific enzyme kinetics (KM and Vmax) of the respective XT isoform for the two peptides were also ...
Similar to SARS-CoV, the cellular receptor of SARS-CoV-2 is angiotensin-converting enzyme 2 (ACE2)3; however, the SARS-CoV-2 spike (S) protein has a 10- to 20-fold higher affinity for ACE2 than the corresponding S protein of SARS-CoV.4 The S prote...
Vaidyanathan et al. Proteolysis of Snap-25 Isoforms by Botulinum Neurotoxin Types A, C, and E: Domains and Amino Acid Residues Controlling the Formation of Enzyme-Substrate Complexes and Cleavage; Journal of Neurochemistry, vol. 72, pp. 327-337. (Year: 1999). ...