4rtilab,affinity biologicals,Allele Biotech,antibodies incorprated,atcc菌种,atcc菌株,berry&Associates,BioAustralis,Biorelevant,Biothema,BMA biomedicals,Briar patch biosciences,Cambridge Isotope Laboratories,Candor Bioscience,cellscript,CIL标准品,click chemistry tools,Complement Technology,Coriell,Creative PEGWorks,Dia...
Cell viability assay and apoptosis assay ALL cells were plated at 1 × 106 cells in six-well plates and treated with the appropriate reagents. Viable and dead cells were counted using trypan blue assay by the TC20 Automated Cell Counter (BioRad Laboratories, Richmond, CA, USA). Apoptosis ...
While this does not rule out the possibility of changes below the limit of detection of our assay, these findings, together with our data that Wnt-induced sensitization to asparaginase can be reversed by direct stimulation of proteasomal activity, support a model in which Wnt-induced sensitization...
Glycerol concentration was estimated by a colorimetric method in the presence of glycerolkinase (GK), glycerol-3-phosphate oxidase (GPO) and peroxidase (HP), using an enzyme-based commercial kit (TL-Labtest®, Biosystem). In this method, the glycerol is phosphorylated to glycerol-3-phosphate ...
equation and plotting 1/[s] and 1/[v] values in the Lineweaver-Burk plot. The turnover number (kcat) ofPg-ASNase II was calculated by using the equationkcat=Vmax/[E0], where [Eo] is the initialPg-ASNase II concentration in the assay andVmax(µmol/min) is the maximum reaction ...
The assay of stability of the system studied in this work has rather complicated history. From the fluorescence measurements of the chemical denaturation of wild type EcA2 and several of its mutants, single transition profile characterised one step highly cooperative unfolding has been observed [3,4...