Asterisks on the x-axis mark proteins that have been identified in each replicate of the iA pulldown but in none of control or iQ replicates and thus represent highly significantly enriched proteins. a, enriched HDACs and proposed off-targets are highlighted (two sided t-test, p value ...
Associated TUG1 RNA was quantified by qPCR using primers indicated in the upper TUG1 scheme. Data are presented as % input, mean ± SD, n = 3. e Left, Western blotting of DHX9 and RPA32 pulled down with TUG1-full length (TUG1) or TUG1-deletion RNA (Δ1-Δ4) indicated...
Jessica B. Spinelli1,2, Haejin Yoon1, Alison E. Ringel1, Sarah Jeanfavre2, Clary B. Clish2, Marcia C. Haigis1* 1.1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. 2.2Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. *↵*Corresponding author. ...
In this paper, we study 2 types of reference points, depending on the timing of elicitation: self-chosen goals, elicited ex ante, by asking students to set their own target grade in a forthcoming exam and post-dictions, elicited immediately after the exam, by asking students to forecast the...
As a counterpoint, the authors do note that even though many molecular biology highly cited articles were done in 1976, there has been extremely much innovation since then however it may take a lot more in experiments and money to gain the level of citations that those papers produced, and ...
“Biology is not simply constrained to the classroom; it is an important facet in everyday life. … I would like my students to face complex cell biology or immunology issues in medicine, research or daily life and be able to assess and solve the problem. I want to give students the too...
the development of highly selective chemical probes for individual bromodomains has helped to elucidate their domain-specific biology and hints towards their unique roles in modulating transcription62,63,64. Understanding the bromodomain-selectivity requirements may provide insights into the therapeutic potenti...
This paper is a portion of the dossier submitted to the FDA for the first (to our knowledge) genetically engineered SCP product to obtain GRAS (generally regarded as safe) recognition by the US Food and Drug Administration. This sets a significant precedent for the advancement of additional, ge...
In conclusion, CHGNet enables charge-informed atomistic simulations amenable to the study of heterovalent systems using large-scale computational simulations, expanding opportunities to study charge-transfer-coupled phenomena in computational chemistry, physics, biology and materials science. ...
A typical protein structure-based drug design (SBDD) project starts from the protein sequence and builds a three-dimensional (3D) structure through structural biology or structure prediction. It then identifies binding pockets, including orthosteric sites or allosteric sites, and finally discovers active...