在真核细胞中，Arf家族GTPase作为分子开关控制膜向内出芽。Arf蛋白在鸟嘌呤核苷酸交换因子(GEF)和GTPase激活蛋白(GAP)控制下实现在激活和关闭状态之间切换。在与GTP结合的激活状态下，Arf特异性地与膜结合，并招募下游coat效应蛋白(如COPI、COPII等)，驱动膜向内出芽形成运输囊泡。图1 向内出芽在真核细胞内膜系统建立...
在真核细胞中,Arf家族GTPase作为分子开关控制膜向内出芽。Arf蛋白在鸟嘌呤核苷酸交换因子(GEF)和GTPase激活蛋白(GAP)控制下实现在激活和关闭状态之间切换。在与GTP结合的激活状态下,Arf特异性地与膜结合,并招募下游coat效应蛋白(如COPI、COPII...
The small GTPase Arl8, a member of Arf-like (Arl) family of proteins, has recently emerged as a crucial regulator of lysosome positioning and membrane trafficking toward lysosomes. Through interaction with its effector SKIP, the human Arl8 paralog (Arl8b) mediates kinesin-1 dependent motility ...
Setty SR, Strochlic TI, Tong AH, Boone C, Burd CG (2004) Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p. Nat Cell Biol 6: 414-419.Setty, S.R.G., Strochlic, T.I., Tong, A.H.Y., Boone, C., and Burd, C....
这是一个令人惊讶的发现,因为传统的想法是,通过增加GTP结合的Arf GTPase,删除ArfGAP蛋白会增加外壳蛋白与膜的结合。然而,我们发现在某些条件下,ArfGAP2的功能独立于GAP活性。然而,ArfGAP2可能独立于GAP活性调节Arf1和AP1,类似于ArfGAP1。我们推测ArfGAP2可能在不同的细胞类型中具有不同的GAP依赖和GAP独立功能。
The GTPase Arl3p is required to recruit a second GTPase, Arl1p, to the Golgi in Saccharomyces cerevisiae. Arl1p binds to the GRIP domain, which is present in a number of long coiled-coil proteins or `golgins'. Here we show that Arl3p is not myristoylated like most members of the Arf...
The GTPase Arl3p is required to recruit a second GTPase, Arl1p, to the Golgi in Saccharomyces cerevisiae. Arl1p binds to the GRIP domain, which is present in a number of long coiled-coil proteins or 'golgins'. Here we show that Arl3p is not myristoylated
Munro S (2005) The Arf-like GTPase Arl1 and its role in membrane traffic. Biochem Soc Trans 33: 601–605 PubMedCASGoogle Scholar Nakano A, Muramatsu M (1989) A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus. J Cell Biol...
有关Arf GTPase及其编码基因的研究工作报道很少,对与之相互作用的调控蛋白研究进展也刚刚开始。主要阐述小G蛋白Arf在动植物及酵母中的研究进展,旨在揭示小G蛋白Arf作为“分子开关”具有重要的细胞生理作用。 关键词小G蛋白;Arf;信号转导 G蛋白是普遍存在于真核生物细胞中的一个GTP结合蛋白家族,根据其亚基组成及分子...
Arf6, like other small G-proteins, cycles between the inactive GDP-bound and the active GTP-bound states. The preferential association of effector proteins with the GTP-bound over the GDP-bound state of Arf6 provides the basis for Arf6’s function in the cell. This highly specific ...