DESIGN/METHODS: EHBL of an NMO patient was examined by CD68, glial fibrillary acidic protein (GFAP), CD3, CD20, neurofilament, Aqp-4 and Aqp-1 antibodies and luxol fast blue staining. Patient was found to be seronegative for Aqp-4-Ab (by cell-based assay) and seropositi...
Secondary Antibodies 同型对照 WB内参抗体 蛋白电泳 WB转印膜组 WB底物 Labeling Kits WB Catalog # 31458 Invitrogen Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP Price (CNY) 2,424.00 Unit Size : 500 µL 添加到购物车 Catalog # A11008 Invitrogen Goat ...
Patterns of antibody binding to aquaporin-4 isoforms in neuromyelitis optica. PLoS One 2010; 5: e10455. 76 Bennett JL, Lam C, Kalluri SR, Saikali P, Bautista K, Dupree C, et al. Intrathecal pathogenic anti-aquaporin-4 antibodies in early neuro- myelitis optica. Ann Neurol 2009; 66: ...
(NMO).Objective:The purpose of this study was to describe the clinical characteristics and temporal dynamics of MOG-IgG in AQP4-IgG seronegative pediatric patients presenting with definite NMO.Methods:Children with definite NMO who were referred for further testing of serum antibodies for AQP4 and ...
Sections were stained with antibodies against AQP4 to show astrocytes (brown) and counterstained with hematoxylin to show nuclei (blue). h For each NMO-IgG preparation used, the phage display peptide library Ph.D.-12 was subjected to three rounds of negative selection on human control-IgG (...
Editorial Does AQP4-Seronegative Neuromyelitis Optica Exist? Michael Levy, MD, PhD JAMA Neurology Comment & Response Features of Neuromyelitis Optica Spectrum Disorders With Aquaporin-4 and Myelin-Oligodendrocyte Glycoprotein Antibodies Jiguo Gao, MD; Wei Pan, MD; Hongliang Zhang, MD, PhD JAMA Neurolog...
Mechanisms of disease: aquaporin-4 antibodies in neuromyelitis optica. Nat Clin Pract Neurol 2008;4(4):202–14. Sign in to download full-size image Figure 8.2. Schematic of AQP4 distribution at the “tripartite” synapse. In addition to its well-known perivascular location on astrocyte endfeet...
Next, the cells were fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.3% Triton X-100 in PBS for 10 min, blocked with 10% goat serum for 20 min, and then incubated with primary antibodies overnight. We used rabbit polyclonal anti-AQP4-IgG (Merck Millipore, Darmstadt, Germany...
We aimed to investigate the frequency and clinical significance of antibodies to myelin oligodendrocyte glycoprotein (MOG-abs) in patients who presented with a first episode of seronegative aquaporin 4 antibody (AQP4-ab) longitudinally extensive transverse myelitis (LETM). Epidemiological, clinical, and...
Patients receiving monoclonal antibodies (mostly B cell-depleting therapies) as their initial and sole therapy throughout disease demonstrated higher likelihood of AQP4-IgG seroreversion compared to those treated with non-specific immunosuppressants (HR 3.01, 95% CI 1.23–7.34,P=0.016) or those ...