Wei L, Xiao M, Hayward A, Fu D (2013) Applications and challenges of next-generation sequencing in Brassica species. Planta 238:1005-1024Wei L J, Xiao M L, Hayward A, et al. Applications and challenges of next - generation sequencing in Brass# ca species[J]. Planta,2013 ,238 :1 ...
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores
Generation of antibody diversity Antibody, also known as immunoglobulin (Ig), is the molecule produced by B cell that can specifically recognize and neutralize a variety of antigens. Antibodies are encoded by a very unique protein-coding system, in which somatic rearrangements happen in the coding ...
Clinical applications and challenges of metagenomic next-generation sequencing in the diagnosis of pediatric infectious disease Infectious diseases seriously threaten the lives of children. Timely and accurate detection of pathogenic microorganisms and targeted medication are the keys to the diagnosing and trea...
Demand for fast, inexpensive, and accurate DNA sequencing data has led to the birth and dominance of a new generation of sequencing technologies. So-called "next-generation" sequencing technologies enable rapid generation of data by sequencing massive amounts of DNA in parallel using diverse methodol...
Recent advances in sequencing and array technologies, which are capable of screening DNA methylomes genome-wide at high-resolution, gave unexpected novel insights in cancer biology. They will be crucial for DNA methylation profiling and the identification of epigenetic biomarker for diagnosis, prognosis ...
Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS lications in basic and lied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and ...
The Application of Single-Cell Sequencing in Dynamic Transcriptomes a timely review on basic principles of single-cell RNA sequencing methods and discuss the technical challenges and their applications in research and medicine... Wang,Xiangdong - Springer Netherlands 被引量: 2发表: 2015年 Reusability ...
However, there are still challenges with the genomic technology. Some tissues of human and animals, such as tumor tissues, contain multiple heterogeneous cells, making analysis extremely difficult. Additionally, some specimens have very few cells, such as circulating tumor cells. To fully study DNA ...
of Animal and Plant Sciences, University of Sheffield, Sheffield, UK As most biologists are probably aware, technological advances in molecular biology during the last few years have opened up possibilities to rapidly generate large-scale sequencing data from non-model organisms at a reasonable cost....