Department of ChemistrySoreyDepartment of ChemistryTimothy L.Department of ChemistryKrollDepartment of ChemistryTodd T.Department of ChemistryJournal of Chemical EducationCarter, J., Petersen, B. P., Printz, S. A., Sorey, T. L. & Kroll, T. T. Quantitative Application for SDS-PAGE in a ...
SDS-PAGE of Proteins Using a Chameleon-Type of Fluorescent Prestain ProteinsFluorescent DyesPeptide MappingElectrophoresis, Polyacrylamide GelStaining and LabelingBlotting, WesternElectrophoresis, Gel, Two-DimensionalSpectrometry, Mass,... RJ Meier,MS Steiner,A Duerkop,... - 《Analytical Chemistry》 被引...
A 2-D native-PAGE/SDS-PAGE method for detecting the subunit components of protein oligomers at low picomole sensitivity is presented. IgG was electrophoresed in a native acidic polyacrylamide gel in amounts ranging from 51 pmol to 60 fmol. Silver-staining (native fast silver stain, ammoni...
and Application]]>Definition, Western Blot
Chinese Spring and its -tetrasomic lines were used to identify the specific bands of STS-marker and microsatellites (SSR) marker of wx genes.Twelve varieties and five waxy wheat lines were screened with these two markers,and the results are in agreement with those from Wx subunits SDS-PAGE. ...
To be able to validate JBoss XML configuration files (such as standalone.xml, domain.xml, and so on) when editing them in IntelliJ IDEA, you can register XML schemas (XSDs) available in the JBoss installation: Click Register schemas. To register all the schemas, just click OK in the dia...
LysG-containing fractions were determined by SDS–PAGE, pooled and dialyzed overnight in 20 mM Tris-HCl pH 8, 500 mM NaCl, and 0.1 mM DTT. The protein was concentrated to 10 mg mL−1 using Amicon Ultra-4 30 K centrifugal filters (Millipore Corporation, Billerica, MA, ...
and for various times using Annexin V-FITC/PI staining (a) and a colorimetric WST-1 assay (b) (n = 3). (C) The PDGF-induced phosphorylation of PLCγ1, STAT3, ERK1/2, Akt, p38, and JNK was measured using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and ...
subtilis 6051a were checked by SDS-PAGE, KstD enzymatic assay and whole-cell biotransformation of 4HP and AD. Whole-cell steroid biotransformation with E. coli BL21 (DE3) and B. subtilis 6051a recombinants The recombinant strains for heterologous expression of kstD genes were cultured in LB ...
Fig. 1: Construction of the peach mAb library and screening of differentially expressed proteins in ripening peach fruit with a mAbArray chip. a Peach protein antigens used to generate the mAb library. SDS-PAGE analysis of proteins extracted from peach fruit that were used as antigens for anti...