十二烷基硫酸钠聚丙烯酰胺凝胶电泳,也称SDS-PAGE,是一种被广泛使用,仅根据分子量大小来分离蛋白质混合物的技术。 阴离子去污剂SDS,在变性的线性蛋白表面沿长度均匀分布使其带电。 将它们上样到聚丙烯酰胺凝胶后, 施加电压,这些表面覆盖SDS的蛋白将被分开。电场作为驱动力,牵引SDS结合的蛋白朝阳极移动,分子量大的蛋白...
Singh N K,Shepherd K W,Cornish G B.A simplified SDS-PAGE procedure for separating LMW Subunits of glutenin.Journal of Cereal Science. 1991SINGH, N.K.; SHEPHERD, K.W.; CORNISH, G.B. A simplified SDS‑PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science...
Western blot is performed by using polypropylene gel electrophoresis. SDS-PAGE allows protein samples to be separated and transferred to a solid support, such as nitrocellulose (NC) or polyvinylidene difluoride (PVDF) membrane. The solid support can absorb the protein and keep its biological activity...
aWe reasoned that the denaturing gel running conditions of SDS-PAGE could impede a robust quantification of KCC2 oligomeric levels, so we subsequently chose to resolve changes in the monomer:oligomer ratio using a previously established nondenaturing PFOPAGE 我们原因SDS-PAGE的弈质的胶凝体运行条件可能...
astained SDS–PAGE for each of the purified His-3xFlag-calnexin[translate] a我不怕痛的 I do not fear the pain [translate] aBest Original Game 最佳的原始的比赛[translate] asoybean in 4-day-old and in the second week of growth in 14-[translate] ...
SDS-PAGE followed by in-gel digestion (IGD) is a popular workflow in mass spectrometry-based proteomics. In GeLC-MS/MS, a protein lysate of a biological sample is separated by SDS-PAGE and each gel lane is sliced in 5–20 slices which, after IGD, are analyzed by LC-MS/MS. The data...
of purification is about 60%.The purified protein was characterized by 16.5% Tricine SDS-PAGE,HPLC,and mass spectrometry,and the molecular mass of the expressed products is in accordance with the cumulated value.HMPIDesB30 can be secretorily expressed by Pichia pastrois.The composition of the ...
on DEAE-cellulose and Sephacryl S-200 columns, and finally by electrofocusing with carrier ampholite. The overall recovery of the hydrogenase was 21% compared with 6% of the previous method. Purified enzyme gave 1 band upon SDS-PAGE. [on SciFinder(R)]Yagi...
Solubilized coat protein components were separated on SDS-PAGE and the desired protein was recovered from the gel pieces under the optimal condition examined. Two purified polypeptides with molecular weights of about 40 kDa were obtained; each of them was in very closed size on SDS-PAGE, both ...
(HOC) PROBE HYBRIDIZATION SOP-NO: MET014_00 Page 5 of 5 4.4 Washing Slides after hybridization 4.4.1 Prepare a low stringency wash buffer I (~500mL) containing 2x SSC and 0.1% SDS 4.4.2 Prepare a low stringency wash buffer II (~500mL) containing 1x SSC 4.4.3 Prepare a high-...