Application of RT-PCR in formalin-fixed and paraffin-embedded lung cancer tissues. Acta Pharmacologica Sinica . 2010; 31 (1):111–117.Zhang F, Wang ZM, Liu HY, Bai Y, Wei S, Li Y, et al. Application of RT-PCR in formalin-fixed and paraffin-embedded lung cancer tissues. Acta ...
320例肠道、肛门、咽拭子样本,应用RT-PCR技术进行研究,取得较好的成 效,现将结果总结如下。 1资料与方法 1.1一般资料 选取我院2013年1月~2014年1月由门诊收入的200例疑似 手足口病患儿作为研究对象,随机选取其肠道、肛门、咽拭子样本共320例。 手足口病的诊断参照我国卫生部2011年修订的手足口病的预防控制指南...
The specificity of the method was also very well, it reduced the possibility of contamination during PCR and false positive results. 展开 关键词: classical swine fever virus (CSFV RT PCR ELISA molecular diagnosis 出版时间: 2003/01/21 ISBN: 1000-6419 ...
立的RT —PCR 方法是一种快速、准确、简便的检测工具,适用于对 BVDV 的大规模流行病学调查、疫 情监测及临床快速诊断. 参考文献 : [1] 殷震 ,刘景 华 .动物病毒学 [M] .第二版 .北京 :科学 出版 社,1997,645 —650 . [2] OIE .& ne vkal diarrlx~ [M].In:Manml of diagn~ tic test...
【Objective】 The chief purpose of this study was to establish A RT-nested PCR assay for detecting Canine distemper virus(CDV).【Method】The primers were designed by using nucleocap sid protein(NP) gene information of CDV Onderstepoort strain in GenBank.The repeatability,specificity and sensitivity...
Three 1-tube Reverse Transcriptase Polymerase Chain Reactions (RT-PCR) directed against the genes encoding the nucleoprotein (NP) and the H5 and H7 hemagglutinin (HA) gene, respectively, were used for detection of avian influenza virus (AIV) in various specimens. A total of 1,040 samples origi...
Real-time PCR (RT-PCR) allows each cycle of DNA amplification to be observed on a computer screen throughout the sequence of thermal cycling, hence the des... Levin,Robert,E. - 《Food Biotechnology》 被引量: 97发表: 2004年 The application of real-time PCR to the identification, detection...
The redundancy of plant genomes leads to large gene families,and the expression analysis of multi-members of a gene family sometimes is critical to understand the exact roles of a gene in plants.Real-time quantitative PCR(RT-QPCR)is an effective method to evaluate gene expressions with satisfacto...
Development and application of RT-PCR assay of Japanese encephalitis virus Objective:To establish an RT-PCR assay for detection of Japanese encephalitis virus(JEV).Mehtods:A pairs of primers specific to capsid protein C gene of JE... ZQ Gao,BF Yue,HE Zheng-Ming - 《Chinese Journal of Pharm...
RT-PCR;病原学检测兔出血病 俗称兔瘟 是由兔出血病病毒 Rab-bithemorrhagicdiseasevirus RHDV 引起兔的一种以呼吸系统出血 实质器官水肿 淤血及出血为主要特征的烈性传染病 易感兔死亡率高达40% 90% 是兔的一种毁灭性疾病 1 本病目前已呈世界性分布 给养兔业带来重大的经济损失 2-5 RHDV属于杯状病毒科兔...