Primer design and validation A primer set of 16 key genes related with different pathways was designed using Primer3 v.4.1.0. The criteria included were primer design in the exonic region of each gene and PCR products between 90 and 250 bp in size. A list of genes and primers is summ...
In LX-2 cells, SP600125 effectively attenuated VP-16-induced apoptosis, as shown by Annexin V staining and the CCK-8 assay, resulting in diminished concentrations of the apoptotic marker protein cleaved PARP and caspase-3. Thus, these findings strongly indicate that ER stress is a crucial ...
It is preferred that the vector harboring the heterologous nucleic acid sequence also contain one or more selectable marker genes so that the transformed cells can easily be selected from non-transformed cells in culture, as described herein. Those of ordinary skill in the art will be able to...
the AST group exhibited a significant increase in serum SOD (P = 0.013) and TAC (P = 0.030), along with a significant reduction in serum MDA (P = 0.005). However, the placebo group showed no significant changes in marker levels...
Therefore, AMH is emerging as a novel ovarian test marker involved in the pathogenesis of POF. Currently, the prevention and treatment of POF are extremely difficult. The most commonly used hormone replacement therapy cannot effectively recover ovarian function [7]. Mesenchymal stem cells (MSCs) ...
The absor- bance was measured at 405 nm with an enzyme marker. 2.7. Reverse Transcription-Polymerase Chain Reaction (RT- PCR) Analysis. The mRNA expression of activating tran- scription factor- (ATF-) 6 and Krüppel-like factor- (KLF-) 6 in HLE-B3 cells was analyzed using an ABI 7500...
phases, (M/T-cell marker). and genes into HEK293 cells in tradition (Fig.?7a). In this system, the secreted Sema7A interacts with PlxnC1 indicated within the HEK-cell surface (Fig.?7b). To analyze the aggregation of PSD, cells had been transfected with the and genes were introduced ...
position of TCA cycle; glutamate dehydrogenase (GLUD) and glutaminase (GLS1) in glutaminolysis; thymidylate synthase (TYMS) and thymidine kinase (TK1) in essential entry position of dTTP synthesis; cleaved poly ADP-ribose polymerase (PARP) and phosphorylated H2AX (γH2AX) as apoptosis marker. Origi...
(for CpG islands prediction and primer design), the miR-301a-5p promoter sequence showed the presence of one CpG islands (Figure 5a), and then carried out a chromatin immunoprecipitation (ChIP) assay to determine the interaction of MBD2 with miR-301a-5p promoter region in HK-2 cells. As...
expression. Based on these findings, HSF2 can be used for marker-assisted selection to modulate the expression of the DDIT3 gene in dairy cattle to improve milk production. It has been widely reported that DDIT3 affects ER stress and induces cell apoptosis or autophagy [21,22,23], but its...