cells that are considered viable are FITC Annexin V and PI negative; cells that are in early apoptosis are FITC Annexin V positive and PI negative; and cells that are in late apoptosis or already dead are are both FITC Annexin V and PI positive. This assay does not distinguish between cell...
诺唯赞试剂说明书 凋亡检测 TUNEL FITC Apoptosis Detection Kit.pdf,TUNEL FITC Apoptosis Detection Kit Vazyme Biotech Co., Ltd 网站/Web: w 咨询热线/Tel: 400-600-9335 w w 销售/Sales: sales@ Vazyme biotech co., ltd. . v a 技术支持/Support: support@ z y m 技术
BD细胞凋亡试剂盒(FITC标记)FITC Annexin V Apoptosis Detection Kit I Materials 1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 μM) to 1 x 10^6 Jurkat cells. 2...
1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in DMSO. 2. Jurkat T cells (ATCC TIB-152). Procedure 1. Add Camptothecin (final conc. 4-6 μM) to 1 x 10^6 Jurkat cells. 2. Incubate the cells for 4-6 hr at 37°C. ...
Flow Cytometric Analysis of Annexin V-PE staining.Jurkat cells were untreated (left panels), treated for 5 hours (middle panels) or 18 hours (right panels) with 4μM Camptothecin (Sigma,Cat.No C9911). Cells were incubated with Annexin V-PE and 7-AAD and analyzed by flow cytometry. Untre...
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PE Annexin V Apoptosis Detection Kit I Product Information Material Number: 559763 Component: 51-66121E Description: 10X Annexin V Binding Buffer Size: 50 ml (1 ea) Storage Buffer: Aqueous buffered solution containing no preservative. Component: 51-68981E Description: 7-AAD Size: 2.0 ml (1 ...
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detection kit (Trevigen, Gaithersburg, MD); (2) fluorescein-based TUNEL was visualized by a cell death detection kit (Sigma-aldrich, St. Louis, MO); and (3) caspase activation was evaluated by incubating cells with CellEvent Caspase-3/7 Green Detection Reagent for 30 min prior to ...
Cell apoptosis was evaluated by Annexin V/PI staining assay and analyzed using an apoptosis detection kit (Keygen, Nanjing, China)37. Briefly, H292 cells were cultured at the density of 1 × 106cells/well. After 24 h, the cells were administrated with lonchocarpin (10 μM) and ...