Figure 3. Fluorescence Flow Cytometry analysis of CaspACETM FITC-VAD- FMK In Situ Marker-labeled apoptotic Jurkat cells. Human Jurkat cells were treated with 100ng/ml anti-Fas antibody for 4 hours, or left untreated, prior toincubation with 10碌M CaspACETM FITC-VAD-FMK In Situ Marker. ...
When it comes to utilizing flow cytometry for apoptosis, Wilensky says that although the tools have remained largely the same, the ability to multiplex probes has become easier. “Instrumentation with more lasers and more fluorescent channels has made it easier to spread out fluorochromes, thus fa...
Figure 3. Fluorescence Flow Cytometry analysis of CaspACETM FITC-VAD- FMK In Situ Marker-labeled apoptotic Jurkat cells. Human Jurkat cells were treated with 100ng/ml anti-Fas antibody for 4 hours, or left untreated, prior toincubation with 10µM CaspACETM FITC-VAD-FMK In Situ Marker. ...
The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm1 and 2800 cm1, a "marker band" of the ...
G. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 53, 3976–3985 (1993). CAS PubMed Google Scholar Tsujimoto, Y., Finger, L. R., Yunis, J., Nowell, P. C. & Croce, C. M. Cloning of the chromosome break...
In general, activation marker expression was negligible on Annexin V**+ cells. This assay allows for a more detailed determination of the cellular immune response of cattle. 展开 收藏 引用 批量引用 报错 分享 全部来源 求助全文 ars.usda.gov 相似文献Flow Cytometric and Fluorometric Methods of ...
The reduction in apoptosis induced by Robo1/21/2 knockout was verified by IHC against caspase-3 (Fig. 5D–E), which is another marker for apoptosis. This is because granulosa cells, which comprise the layer of small cells that form the wall of the ovarian follicle, are fundamental in ...
As a measure of the NP formulation's efficacy in vivo, IL-17A (as an inflammatory marker) was assayed and it was determined that the IL-17A was significantly decreased [77]. 4.1.2 Clinical studies Several clinical studies have been conducted with NP formulations to determine whether there is...
(PARP), a nuclear enzyme of DNA repair that responds to DNA damage can be cleaved by one apoptotic effector caspase 3. The cleavage of PARP means the dysfunction of the enzymatic DNA repair function, which has been researched in detail as a apoptosis marker32. Cell cycle analysis indicated ...
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