Application of flow cytometry to the study of cell death has three goals: identification and quantification of dead and dying cells; discrimination between apoptotic and necrotic modes of cell death; and elucidation of mechanisms involved in cell death. This massively detailed unit by a pioneer in ...
Apoptosis & Necrosis Assay (Flow Cytometry) Detect compound induced cell death using Cyprotex’s flow cytometric apoptosis and necrosis assay. Cyprotex delivers consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements. Introduction Background information...
Use of a fluorochrome, such as PI, that is capable of binding and labeling DNA makes it possible to obtain a rapid (the protocol can be completed in about 2 h) and precise evaluation of cellular DNA content by flow cytometric analysis, and subsequent identification of hypodiploid cells. ...
Figure 6. Analysis of cell viability and apoptosis by flow cytometry. Experimental protocol to study cell viability and apoptosis Adherent cells: Remove medium and wash cell monolayer with PBS Add dissociating agent (e.g., trypsin) and incubate at 37°C for 5 min or until cells d...
To do this, we used an improved sample-processing technique and a large-volume amplification protocol. The objective of this study was to use advanced molecular techniques to develop a rapid and simple method which can be used by the water industry for detection of viral contamination in a ...
the pro-apoptotic proteins like BAX and BAK initiate the mitochondrial pathway leading to apoptosis. Below is a western blot analysis that confirms antibody specificity of a Bcl-2 antibody that would be suitable for apoptosis research and a flow cytometry analysis of Mc...
Finally, know when another protocol is more appropriate. The target will inform the approach. “Western blotting is often used to detect cleavage of caspases or apoptosis target proteins like Bcl-2 family members, where the antibody can't distinguish cleaved or uncleaved protein unless the proteins...
Figure 6. Analysis of cell viability and apoptosis by flow cytometry. Experimental protocol to study cell viability and apoptosis Adherent cells: Remove medium and wash cell monolayer with PBS Add dissociating agent (e.g., trypsin) and incubate at 37°C for 5 min or until cells detach...
Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat Protoc 1: 1458-1461 Since its introduction, the propidium iodide (PI) flow cytometric assay has been widely used for the evaluation of apoptosis in different experimental mode... C Riccardi,I Nicoletti - 《Nature Protocol...
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies. Request the handbook Imaging protocol handbook Protocols that fit your needs in imaging ranging from sample and assay preparati...