Detect compound induced cell death using Cyprotex’s flow cytometric apoptosis and necrosis assay. Cyprotex delivers consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies. Request the handbook Imaging protocol handbook Protocols that fit your needs in imaging ranging from sample and assay preparati...
A novel assay for apoptosis. Flow cytometric detection of phos- phatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 1995; 184:39-51.Gorczyca W, Melamed MR, Darzynkiewicz Z (1998) Analysis of apoptosis by flow cytometry. Methods Mol Biol ...
Since its introduction, the propidium iodide (PI) flow cytometric assay has been widely used for the evaluation of apoptosis in different experimental models. It is based on the principle that apoptotic cells, among other typical features, are characteri
Below is a description of the more common assays that can be used with flow cytometry. Annexin V Assay: The flipping of phosphatidylserine (PS), from the inner to the outer leaflet of the cell membrane is an early to mid phase signal of apoptosis. This assay relies on Annexin V binding ...
Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat Protoc 1: 1458-1461 Since its introduction, the propidium iodide (PI) flow cytometric assay has been widely used for the evaluation of apoptosis in different experimental mode... C Riccardi,I Nicoletti - 《Nature Protocol...
Our annexin V conjugates are available as stand-alone reagents, each suitable for 50–100 flow cytometry assays or many more imaging assays, or in several variations of our apoptosis assay kits (Molecular Probes apoptosis assay kits—...
This is an example protocol for PS exposure detection using Annexin V, based on the protocol provided inAnnexin V-FITC Apoptosis Detection Kit (ab14085).Please be aware that when working with a specific kit, you should always use the protocol provided on the datasheet as it has been designed...
Flow cytometry Routinely Tested Recommended Assay Procedure: PE Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. PE Annexin V bind...
cells in culture. Cytometry. 1996; 24(2):131-139. (Biology) Vermes I, Haanen C, Steffens-Nakken H, Reuingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995; ...