Mix and match our standalone reagents or choose from our selection of multi-parametric apoptosis assay kits that are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single ...
Find an apoptosis assay. Use a selection guide. Choose from annexin V conjugates, the JC-1 reagent, caspase assays, TUNEL assays, mitochondria probes & more.
10- N -Nonyl acridine orange (NAO), a fluorophor that forms a stable complex with reduced form of CL, but not with oxidized CL, can be used for flow cytometry analysis of this effect. It has been shown that after the incubation of CTLL-2 cells in the presence of 7% ethanol (90 ...
Detect compound induced cell death using Cyprotex’s flow cytometric apoptosis and necrosis assay. Cyprotex delivers consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
经过人们多年的研究发现,应用原位末端转移酶标记技术(TDT assay or TUNEL reaction)来检测细胞凋亡,其灵敏度高,特异性强,能早期显示未发生典型变化的凋亡细胞。它是检测单个细胞早期出现凋亡现象的好方法。近几年,人们又发现了能更快、更敏感、检测细胞数量更多的并能从更多方面证明凋亡和坏死的流式细胞分析术(flow ...
Below is a description of the more common assays that can be used with flow cytometry. Annexin V Assay: The flipping of phosphatidylserine (PS), from the inner to the outer leaflet of the cell membrane is an early to mid phase signal of apoptosis. This assay relies on Annexin V binding ...
凋亡事件3:Caspase activity assay kits / 检测 Caspase 活化 当Cytochrome C释放至细胞质中,会导致Caspase活化,而活化的Caspase会引发一连串的蛋白质酶解作用(proteolysis cascade),将凋亡信息送至更下游的物质,造成细胞的特征性型态改变。 一、Caspase家庭介绍 ...
Apoptosis assay was performed as detailed by [24]. Six well culture plates were considered to grow RAW264.7 cells and the mycobacteria were infected for about 24 h. The apoptotic cell percentage was determined using Annexin V-FITC apoptosis kit and flow cytometry has been performed per 10,000 ...
Flow cytometry Routinely Tested Recommended Assay Procedure: FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10^-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V ...
Find an apoptosis assay. Use a selection guide. Choose from annexin V conjugates, the JC-1 reagent, caspase assays, TUNEL assays, mitochondria probes & more.