1、后期促进复合物/细胞周期体(APC/C):大型、多亚基E3泛素连接酶复合物,在真核细胞周期调控中发挥关键作用。 2、Cdc20:一种关键的有丝分裂调节蛋白,与APC/C结合形成APC/C-Cdc20复合物,在有丝分裂过程中发挥重要作用。Cdc20的过度...
The depletion of endogenous PHD3 in CDC20 knockdown HCC cells greatly attenuated the decline of HIF-1a protein and restored the secretion of VEGF. In contrast, overexpression of a non-degradable PHD3 mutant significantly inhibited the proliferation of HCC cells both in vitro and in vivo. ...
CDC20(cell division cycle20)是后期促进复合物APC/C的两个辅酶因子之一,能在特定时间点结合并激活APC复合物而发挥作用,在指导细胞周期相关蛋白泛素化降解的过程中发挥重要的作用,保证正常的染色体分离。由于大部分的细胞周期相关蛋白都通过泛素化途径进行降解,CDC20在对细胞周期的调控作用起关键作用。然而,目前为止对...
The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex. Nature cell biology. 2004; 6:129-137.Song MS, Song SJ, Ayad NG, Chang JS, Lee JH, Hong HK, Lee H, Choi N, Kim J, Kim H, Kim JW, Choi EJ, Kirschner MW, Lim DS: The tumour suppressor RASSF1...
directed against Cdc20 induced an accumulation of E2F1 protein in prometaphase cells. These data suggest that APC/C(Cdc20) specifically targets E2F1 for degradation in early mitosis and reveal a novel mechanism for limiting free E2F1 levels in cells, failure of which may compromise cell survival ...
APC活性的调节:正调控因子:cdc20/Fizzy,Cdh1/Fzy负调控因子:Emi1、Emi2、Mad2、BubR1等。 Cdc20主要位于染色体动粒上,为姐妹染色单体分离所必需的Mad2正常情况下定位在早中期和错误排列的中期染色体的动粒上,所以纺锤体组装不完全,动粒不能被动粒微管捕捉,mad2则不能从动粒上解离下来。APC的活性还受到纺锤体组装...
The in vivo function of Cdc20 phosphorylation, its exact temporal control and the kinase(s) responsible are, however, largely unknown. Here we show that cyclin A2–Cdk2 phosphorylates Cdc20 on sites close to the C-box in interphase. Cdc20 phosphorylation is required to inhibit the APC/C–...
Regulation of APC-Cdc20 by the spindle checkpoint. Recent studies shed light on how the spindle checkpoint guards genome integrity by sensing the strayed chromosomes in mitosis and blocking the actions of t... H Yu - 《Current Opinion in Cell Biology》 被引量: 732发表: 2002年 Regulation of...
Furthermore, the APCCdc20E3 ligase is validated as the upstream regulator marking TPD52 for polyubiquitination‐mediated proteolysis. In addition, inactivation of Cdc20 sensitized cancer cells to treatment with the ER stress inducer in a TPD52‐dependent manner. Thus, the study suggests that TPD52...
Cdc20(cell division cycle 20 homologue)是一种细胞周期调节蛋白,在很多肿瘤中都有高表达,是关键的促癌因子,对有丝分裂进程特别是肿瘤干细胞的发展至关重要。因此开发特定的Cdc20抑制剂是改善人类癌症治疗的新策略。Apcin是目前唯一已报道的Cdc20特异性抑制剂,但其抗细胞增殖的能力较弱。 2020年4月15日,中南大学...