The antibody phage display system has been investigated as a vehicle for the potential altering of a catalytic antibody's specificity and chemical reactivity. Using previously identified catalytic antibodies, heavy and light chain shuffling experiments have been conducted. Catalytic activity and specificity...
Antibody-based research applications are critical for biological discovery. Yet there are no industry standards for comparing the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed and approved jointly by industry and academ...
Several key residue substitutions were identified in NGS data with high-affinity enrichment and selectivity for multiple FP antigens, and were selected for expression as soluble IgG for additional study (Fig. 2A, Supplemental Table 2 single-mutant variants identified by yeast-library screening). We ...
Selectivity, reproducibility and stability of the four immunosensors. The selectivity of GE-G- Chi-Au/PtNP-Glu-ARV/MAb-BSA, GE-G-Chi-Au/PtNP-EDC/NHS-ARV/MAb-BSA, GE-G-Chi-Au/PtNP- ARV/MAb-BSA and GE-G-Chi-Au/PtNP-CH-ARV/MAb-BSA played a crucial role in detecting target ...
Antibodydrug conjugate(ADC) is a type of combinatorial drug delivery that simultaneously takes advantage of the tumor selectivity and specificity of conjugatedmonoclonal antibodiesas well as the cell-killing efficacy of thecytotoxic drugs[73]. By utilizing monoclonal antibodies as targeting moieties ...
Many advances in higher sensitivity, selectivity, and novel attachments for various functions have been recently developed for the POCT of respiratory viruses. Immunoassay has advantages in rapid results, easy to use and cost-effective platform. Current disadvantages are limited sensitivity, cross-...
Antibody–drug conjugates (ADCs) are a relatively new class of anticancer agents designed to merge the selectivity of monoclonal antibodies with cell killing properties of chemotherapy. They are commonly described as the “Trojan Horses” of therapeutic armamentarium, because of their capability of direc...
Heavy-Chain Complementarity-Determining Regions Determine Conformation Selectivity of Anti-A beta Antibodies. Neurodegener Dis. 2011;8:117–23. Article CAS PubMed Google Scholar Kissel K, Hamm S, Schulz M, Vecchi A, Garlanda C, Engelhardt B. Immunohistochemical localization of the murine ...
Since antibody repertoire sizes for an individual range from 103 for zebrafish to 1010 for humans9, 10, computational approaches represent a practical structure-based method to study antibody specificity and selectivity. To understand the driving forces behind the generation and maturation of these ...
In order to precisely evaluate the selectivity for toxic conformer of Aβ4 2, the concentration of clones used in this re-eval- uation (15~120 ng/mL) was lower than that of the previous study13 (125~1,000 ng/mL). Enzyme immunoassay (EIA) identified a unique clone, named 24...