Mochly-Rosen D, Gordon AS (1998) Anchoring proteins for protein kinase C: a means for isozyme selectivity. FASEB Journal 12:35–42 Google Scholar Staudinger J, Lu J, Olson EN (1997) Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-α. Jour...
In order to precisely evaluate the selectivity for toxic conformer of Aβ4 2, the concentration of clones used in this re-eval- uation (15~120 ng/mL) was lower than that of the previous study13 (125~1,000 ng/mL). Enzyme immunoassay (EIA) identified a unique clone, named 24...
We examined the Gβ and Gγ subunits interacting with α2aARs to distinguish which Gβ and Gγ subunits interact with auto- vs. hetero-α2aARs. In Figs2and3, we applied the quantitative MRM method28to co-IP samples of wildtype (WT) and HA-α2aARs mouse synaptosomes. Using SDS-PAGE ...
Gitlin et al., “The selectivity of the human placenta in the transer of plasma proteins from mother to fetus,” J Clin Invest 43:1938-1951 (1964). Giusti et al., “Somatic diversification of S107 from an antiphosphocoline to an anti-DNA autoantibody is due to a single base change in...
CHO vs. HEK-293, in Western blots, none of them produced a distinct band in the cells expressing their target receptor as compared to those expressing related subtypes. While these findings raise questions about the selectivity of the antisera for their respective cognate receptors, several caveats...
Team 'Endocannabinoids and Neuroadaptation', Bordeaux, France; 2Université de Bordeaux, Bordeaux, France; 3Department of Physiology and Pharmacology, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada Endocannabinoids (eCBs) are amongst the most ubiquitous signaling molecules in the ...
It is known that lipophilicity can play a crucial role [20] and a summary of substrates/selectivity rules is proposed [21]. However, there is an urgent need to improve the accuracy, interpretability and confidence of the computation models used in drug discovery process (see [19] and ...
Posteriorly, a toxin named Tf2 was electrophysiological characterized using two-electrode voltage-clamp technique on human sodium channels subtypes, showing high selectivity to NaV 1.3 [20]. This work aims to describe Tf1a and its activity on NaV1.1–1.7, Bg NaV, and Vd NaV by ...
generated soluble HA protein from JX346 using a baculovirus expression system and then assayed the sialyl glycan selectivity of the HA using a solid-phase platform, surface plasmon resonance (SPR) and tissue staining platforms [82]. The SPR data revealed that the HA of the H10N8 virus JX346...