In addition to LNPs, polymer-based PLGA-based NPs have also been investigated for the delivery of drugs via antibody conjugates. For example, Hu et al. tested the paclitaxel-carrying PLGA conjugated antibody against the carcinoembryonic antigen overexpressed in 90% pancreatic tumo...
where an antibody is conjugated via a ligand with functionalized LNPs carrying cytotoxic drugs; and two, where an antibody is conjugated directly with drug through a linker at the specific site. Let’s consider the latter one first as several ADCs have been approved in the recent past. ...
(SPDP) was conjugated to the side chain of the lysine unit to generate acetal-PEG-PLys-(PDP), and DTT further reduced to produce acetal-PEG-PLys (containing sulfhydryl groups). cRGD receptors (αVβ3 and αVβ5 integrins) are widely expressed on the surface of tumor cells, so this ...
We recommend our CoraLite® Plus 488 conjugated versions of this antibody. 推荐稀释比 ApplicationDilution Western Blot (WB) WB : 1:5000-1:50000 Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC) IHC : 1:50-1:500 Immunofluorescence (...
As of January 2021, four siRNA-based drugs with lipid nanoparticle (LNP) orN-acetylgalactosamine (GalNAc) delivery systems had received regulatory approval from the FDA or European Medicines Agency (EMA).82Encapsulated in LNPs or covalently conjugated to GalNAc ligands, siRNAs can be successfully deli...
(Proteintech, cat. no. 66009-1-Ig), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Servicebio, cat. no. GB25303), Cy5-conjugated goat anti-rabbit IgG (Servicebio, cat. no. GB27303). anti-ERK1/2 (Cell Signaling Technology, cat. no. 4695), anti-p-ERK1/2 (Santa Cruz ...
If a monoclonal antibody is conjugated to a radioisotope that could be detected by a gamma camera, the antibody could be used to visualize tumors. If the same monoclonal antibody is conjugated to an alpha- or beta-emitting radioisotope, it could be used as a therapeutic agent. The therapeutic...
Generation of recombinant and hapten-conjugated proteins Recombinant HAs used for immunizations and ELISAs were produced in-house using a CHO cell protein expression system, as described previouosly21. Cysteine residues were introduced into the HA sequence to create trimer-stabilizing disulfide bonds, ...
The first peak shows conjugated protein. The second peak contains unconjugated RBD. b, Analytical size-exclusion trace shows a homogenous nanoparticle preparation. c, Negative-stain electron microscopy image of RBD–scNP on a carbon grid. Inset shows a zoomed-in image of RBD–scNP. The zoomed ...
HRP-conjugated IgG1 (Southern Biotech, 1144-05), IgG2a (Southern Biotech, 1155-05), or IgG2b (Southern Biotech, 1186-05) were diluted 1:5,000 in blocking buffer and incubated for 1 hour, then washed seven times. Plates were developed with Pierce TMB Substrate (Thermo Scientific, 34021),...