We have evaluated antibody coated beads for capture and detection of carcinoembryonic antigen (CEA). Assay parameters of time, temperature, buffer molarity, specificity of antibody on the bead and reagent addition sequence have been studied. Optimal assay kinetics occurred at a temperature of 45掳C ...
We use anti-CD62P coated beads to capture activated platelets from samples stimulated with a PAR-1 specific agonist SFLLRN, also known as thrombin receptor activator peptide. The responsiveness of the platelets is analyzed via the remaining unbound platelets in the solution and compared to a ...
Dynabeads magnetic beads coated with secondary antibodies. (Dynabeads Pan Mouse IgG magnetic beads (Cat. No. 11041) are 4.5 µm beads developed for cell separation that have increased capacity per volume when using Dynabeads M-280 Sheep Anti-Mouse IgG magnetic beads. Thus, there are several ...
Moisture on unused beads will deactivate the reactive groups necessary for covalent antibody coupling. To avoid condensation on unused beads,make sure the beads are at room temperature prior to opening the bottle. Table 1: Calculation of Antibody and C1 Volumes ...
Cytotoxic T lymphocytes were found to mediate rapid lysis of target cells not normally recognized in the presence of small polystyrene beads coated with a combination of anti-T3 and antitarget cell antibodies. Lysis was not seen with beads bearing one of these antibodies alone, nor with a mixtur...
Briefly, samples were incubated with antigen-coated beads in BAMA assay diluent (5% Normal Goat serum (v/v), 1% milk blotto (w/v), 0.05% Tween-20 (v/v) in PBS), washed, then incubated with either biotinylated anti-human IgG (Southern Biotech) or biotinylated anti-human IgG3 (...
Protein A-coated magnetic beads (Dynabeads, Life Technologies) were saturated with NI301A or anti-TTR Dako A0002 antibodies and incubated for 30 min at room temperature with TTRwt tetramers, or with patient-derived ATTR fibrils functioning as controls, or with human plasma samples diluted 0,...
LC-MS/MS quantification of a signature peptide after trypsin digestion of plasma samples before and after an extraction of the total IgG content of plasma with protein G coated beads was used to determine the total and the ADA-bound fractions of rhGAA in samples from Pompe patients after ...
For each equilibrium binding study, DKK1 or sclerostin at three fixed concentrations was incubated with titrations of the testing molecules (Ab or hetero-DS) at room temperature for at least 8 h before being flowed over Hetero-DS-coated beads. Free DKK1 or sclerostin in the equilibrated ...
We correlated fluorescence intensity with the number of GFP molecules by FACS analysis of beads coated with different and known amounts of GFP. Use of this standard allows determining the number of antibodies in the cytosol from the fluorescence intensity in the cells. To evaluate the fluorescent ...