To test on agar, prepare a bacterial lawn plate as if preparing for a disc diffusion test (Section 4.3). Place the material or dressing directly onto the plate, incubate the plate at about 35°C for 16–20 h and measure the zone of inhibition with callipers as in Section 4.3. Like ...
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As a control, cells were heated to 60 °C for 20 min, which resulted in ~ 57% of the cells being permeable to PI. As shown in Figure 2d, the integrity of the E. coli FRAG-1 membranes was not compromised with concentrations of 10 up to ~ 54 μg ml − 1. However, there was...
12.4E) probably mainly act on the functions of topoisomerases in cells by forming a stable DNA–drug–enzyme complex [43]. Etoposide (an analogue of podophyllotoxin) acts on topoisomerase II, especially when the enzyme is complexed with DNA. The drug reacts only weakly with DNA in the ...
Apply a small amount of this product (an amount equal to the surface area of the tip of a finger) on the area 1 to 3 times daily May be covered with a sterile bandage Children under 2 years of age: ask a doctor Other information Store at 20° to 25°C (68° to 77°F) ...
A and B, conditioned with helium, were used as the mobile phases at a flow of 0.5 ml min − 1 for a total analysis time of 60 min. A linear gradient of 0–100% eluent B was applied and the column temperature was kept constant at 25 ºC. A Waters 2487 dual absorbance detector...
The emergence of antimicrobial resistance in bacterial pathogens is a growing concern worldwide due to its impact on the treatment of bacterial infections. The "Trojan Horse" strategy has been proposed as a potential solution to overcome drug resistance
Computer models show how the bacteriophage T4 (left panel) attacksE. coli, which normally inhabits the gastrointestinal tract of humans. T4’s invasion starts by touching down on the bacterial cell wall and injecting viral DNA through its tube-like tail (purple) into the cell. A “packaging ...
On the homepage, the mascot asks students to choose a theme for training (e.g. respiratory infections). Once the theme has been chosen, the game starts with a first clinical case (Fig. 2). Students have to read thepatient record, and then answer the patient’s questions. The student se...
The aim of this in vitro study was to evaluate the pharmacodynamic effect of erdosteine in combination with antibiotics against biofilms formed by the clinically important bacteria methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) using a model based on that of Ba...