Ambion™ 抗 miR™ miRNA 抑制剂是经过化学修饰的单链核酸,设计用于特异性结合并抑制内源性 microRNA (miRNA) 分子。可使用与 siRNA 所用相似的转染或电穿孔参数将这些即用型抑制剂导入细胞中。Anti-miR™ let-7c miRNA 抑制剂阳性对照为使用 Anti-miR miRNA 抑制剂的实验提供了便利、
为了探讨anti-miRNA-21与肝细胞癌Huh 7细胞对吉非替尼敏感性之间的关系,通过MTT法和流式细胞术检测anti-miRNA-21联合吉非替尼对Huh 7细胞增殖和凋亡的影响.用Western blot检测anti-miRNA-21对同源性磷酸酶-张力蛋白(PTEN)表达的影响,以及联合吉非替尼对ERK/Akt信号通路相关蛋白表达的影响.结果显示,anti-miRNA-...
DOTA-anti-miR PNAs were then labelled with copper-64 (Cu) to function as non-invasive molecular imaging tools. The affinity of the probes for the targets was assessedby circular dichroism and melting temperature. Differential uptake of fluorescein andCu labeled anti-miRNA probes was tested on ...
For example, in breast cancer cells, both miR-148a-3p over-expression through specific miRNA mimics and its inhibition by anti-sense oligonucleotides resulted in reduced cancer cell proliferation [23], [33], [34]. Likewise, both over-expression and knockdown of miR-148a-3p levels have been ...
摘要: 目的 探讨anti-miRNA-155反义寡核苷酸(AMOs)对腹膜成纤维细胞(FB)增殖的作用及可能机制.方法 大鼠腹膜FB细胞分为:实验组(anti-miRNA-155 AMOs 50nmol/L)、阴性对照组(错义链50nmol/L)和空白对照组(PBS).采用Lipofec-tamine2000作为载体分别转染... 查看全部>> ...
METTL1/WDR4复合体将m7G修饰加到mRNA(internal site)、tRNA(G46 位点)和miRNA (G-四联体),最终调控翻译。 RNMT/RAM复合体将m7G加到mRNA的5’帽子上,介导其出核和翻译。 WBSCR22/TMRT112复合体将m7G修饰加到18s rRNA的G1638处,促进其成熟。 发文章突破点:错过了m6A,可以试试m7G,例如挖掘m7G相关基因,构建预...
antimiRNA-10b是一种反义RNA分子,用于抑制miRNA-10b的功能,从而调控与癌症转移、侵袭相关的基因表达,具有潜在的抗肿瘤作用。uPAR修饰负载antimiRNA-10b的乳腺癌细胞外泌体是一种创新的靶向治疗系统,通过在乳腺癌细胞来源的外泌体表面修饰尿激酶型纤溶酶原激活物受体(uPAR),使外泌体能够特异性地靶向乳腺癌细胞。外泌...
Our previous research showed anti-cancerous effects of LCB on HepG2 cells. The integrated miRNA and mRNA omics data generated in the progression of LCB induced cytotoxicity on Human Hepatoma Cell HepG2 can help to comprehend the underlying mechanism which would CRediT authorship contribution ...
THP-1, Kasumi-1, primary CD34+ HSC and primary AML cells were transfected with control miR (miR-mimic negative control and the anti-miR miRNA inhibitor negative control for single experiments and combined miR-mimic negative control and the anti-miR miRNA inhibitor negative control in combined ...
antimiRNA-21是一种小分子反义RNA,用于抑制miRNA-21的功能,从而调控与肿瘤发生、发展和抗药性相关的基因表达,具有潜在的癌症治疗作用。乳腺癌细胞外泌体是由乳腺癌细胞分泌的纳米级囊泡,携带癌症相关分子,如RNA、蛋白质和脂质,参与肿瘤进展、转移以及免疫逃逸,具有重要的生物标志物和治疗潜力。uPAR修饰负载antimiRNA-...