The surface of eukaryotic cells is a multi-component fluid bilayer in which glycosylphosphatidylinositol (GPI)-anchored proteins are an abundant constituent. In this review, we discuss the complex nature of the organization and dynamics of GPI-anchored proteins at multiple spatial and temporal scales....
demonstrated to have the capacity to degrade fibrinogen, to activate pro-uPA,232, 235 to activate membrane receptors such as PAR-2,232, 235, 236, and to cleave the D1–D2 linker sequence of human uPAR (h-uPAR), which prevents interaction with the extracellular matrix component, vitronectin...
the GPI anchor, to the external leaflet of the plasma membrane27. GPI is broadly distributed among eukaryotes, including protozoa, fungi, plants, insects, and mammals, while proteins containing a GPI
All the above mentioned models are, however, derived on the assump- tion of a single, cylindrical membrane domain embedded in a large, homogenous two-dimensional fluid, e.g. a single-component phospholipid bilayer. 1Department of Cell and Developmental Biology, Biocenter, University of Würzburg,...
We show here that COBL10, a glycosylphosphatidylinositol (GPI)-anchored protein, is one component of this pollen tube internal machinery. Mutations in COBL10 caused gametophytic male sterility due to reduced pollen tube growth and compromised directional sensing in the female transmitting tract. ...
Neutralizing antibodies block viral entry by recognizing epitopes on the envelope spike critical for its attachment, receptor and co-receptor interaction, or fusion and appear to be an important component of a protective immune response [1]. However, antibodies that can neutralize a broad range of ...
cerevisiae, though its deletion delays ER-export of GPI-anchored proteins due to its interaction with the p24-complex during COPII trafficking (Manzano-Lopez et al. 2015). TED1 deletion mutants hence resemble deletion mutants of the p24-complex component encoding genes EMP24 and ERV25, which ...
We also examined CpGap1 gene expression under the solid culture conditions supplemented with tannic acid (TA), a well-known and abundant plant defense component in chestnut trees (Dai and Mumper, 2010). TA supplementation significantly changed the accumulation of CpGap1 transcripts in both wild-ty...
Caveolae are plasma membrane specializations present in most cell types. Caveolin, a 22-kDa integral membrane protein, is a principal structural and regulatory component of caveolae membranes. Previous studies have demonstrated that caveolin co-purifies with lipid modified signaling molecules, including G...
Peptides, typically 8-10 amino acids long, bind to a special groove formed between the two membrane-distal domains of the α chain, α1 and α2, mainly via 2-3 dominant anchor residues. CTLs serve as the major effector arm of the immune system and represent an important component of ...