The success of this construct in targeting plasma membrane-anchored fluorescent proteins to SEs could be attributable to the small size of AtRCI2A or to the presence of other signals innate to AtRCI2A that permit the protein to be trafficked to SEs. The construct provides a hitherto unique ...
Since the most significant gradients of endogenous extracellular H2O2 can be observed only in a very shallow region of the fluid in contact with the plasma membrane, we show here the use of a newly designed nanosensor anchored to the outer cell surface and capable of quantifying H2O2 at ...
Plasma Membrane Area Increases with Spread Area by Exocytosis of a GPI-anchored Protein Compartment 作者:Gauthier Nils C; Rossier Olivier M; Mathur Anurag; Hone James C; Sheetz Michael P* 来源:Molecular Biology of the Cell, 2009, 20(14): 3261-3272. ...
This plasma membrane location was blocked in vivo in prenyltransferase mutants and by mevinolin, which inhibits the synthesis of prenyl groups. In addition to the five MUBs with CAAX boxes, Arabidopsis has one MUB variant with a cysteine-rich C terminus distinct from the CAAX box that is ...
Evidence for segregation of heterologous GPI-anchored proteins into separate lipid rafts within the plasma membrane. J Membr Biol 189: 35- 43, 2002.Wang J, Gunning W, Kelley KM, Ratnam M. Evidence for segregation of heterologous GPI-anchored proteins into separate lipid rafts within the plasma ...
reportedphenotypesmightbeexplainedbyamechanisminvolvingtheinteractionwiththesubunit5oftheCOP9signalosome(CSN).Citation:MirR,Leo´nJ(2014)PathogenandCircadianControlled1(PCC1)ProteinIsAnchoredtothePlasmaMembraneandInteractswithSubunit5ofCOP9SignalosomeinArabidopsis.PLoSONE9(1):e87216.doi:10.1371/journal.pone....
The modified proteins are then directed to the PM (plasma membrane). Less well understood is how nascent mammalian GPI-anchored proteins are targeted from the ER to the PM. In the present study, we investigated mechanisms underlying membrane trafficking of the GPI-anchored proteins, focusing on ...
Since the most significant gradients of endogenous extracellular H2O2 can be observed only in a very shallow region of the fluid in contact with the plasma membrane, we show here the use of a newly designed nanosensor anchored to the outer cell surface and capable of quantifying H2O2 at ...
The fundamental mechanisms of protein and lipid organization at the plasma membrane have continued to engage researchers for decades. Among proposed models, one idea has been particularly successful which assumes that sterol-dependent nanoscopic phases of different lipid chain order compartmentalize proteins...
Membrane ProteinsBiological TransportModels, MolecularGlycosphingolipidsGlycosylphosphatidylinositol (GPI)-anchored proteins participate in many cell surface functions; however, the molecular associations of these lipid-linked proteins within the plasma membrane are not well understood. Recent biochemical ...