The construct, called by its abbreviation SUmCR, yielded stable sieve element (SE) plasma membrane fluorescence labeling, even after plastic (methacrylate) embedding. In conjunction with wide-field fluorescence measurements of sieve pore number and position using aniline blue-stained callose, mCitrine-...
摘要 The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area...
This plasma membrane location was blocked in vivo in prenyltransferase mutants and by mevinolin, which inhibits the synthesis of prenyl groups. In addition to the five MUBs with CAAX boxes, Arabidopsis has one MUB variant with a cysteine-rich C terminus distinct from the CAAX box that is ...
Using solution NMR spectroscopy, we obtained the structure of Ca2+-calmodulin (holoCaM) in complex with peptide C28 from the binding domain of the plasma membrane Ca2+-ATPase (PMCA) pump isoform 4b. This provides the first atomic resolution insight into the binding mode of holoCaM to the ...
reportedphenotypesmightbeexplainedbyamechanisminvolvingtheinteractionwiththesubunit5oftheCOP9signalosome(CSN).Citation:MirR,Leo´nJ(2014)PathogenandCircadianControlled1(PCC1)ProteinIsAnchoredtothePlasmaMembraneandInteractswithSubunit5ofCOP9SignalosomeinArabidopsis.PLoSONE9(1):e87216.doi:10.1371/journal.pone....