The falsely detected positive samples were all of low titer (1:80). System A found 157 and System B 156 patterns in agreement to the target pattern. From 80 negative samples system A detected 73 and system B 71 samples correctly.ConclusionsBoth systems resulted in an overall sensitivity >95...
The “gold standard” serologic test for diagnosis of HGA in humans is an indirect immunofluorescence assay (IFA). This assay must be performed at multiple time points over a period of several weeks and can only be performed in specialized reference laboratories. A limitation of the IFA assay ...
phagocytophilum of at least 80; (2) a fourfold increase or decrease in the antibody titer within 4 weeks; (3) a positive PCR test result using specific A. phagocytophilum primers; or (4) isolation of A. phagocytophilum from blood.22 These criteria could also be applied to d...
1 Anti-NuclearAntibody(ANA) 1.ScreeningTest A.Referstoauto-antibodiestonuclear componentsofthecell B.Seeninawidevarietyofsystemicautoimmune diseases C.Usefulasdiagnosticmarkersandtomonitor thecourseofautoimmunediseases D.Canbefoundinlowtitersinhealthy ...
14 patients showed double-positivity by PCR and IFA tests. Twenty-four patients out of 139 had an IgM titer of 1:16 or higher, or an IgG titer of 1:80 or higher in the acute phase, but there was no increase in antibody levels in the recovery phase. In addition, when PCR forAnaplas...
Immunodominant epitope: The epitope on a molecule that induces a dominant, or most intense, immune response. The immunodominant epitope may elicit, for example, the greatest antibody titer during infection or immunization, as measured by, for example, the fraction of reactivity attributable to a cer...