Use of Computer-Aided Immunofluorescence Microscopy (CAIFM) for Interpretation of Antinuclear Antibody (ANA) Pattern and Titerantinuclear antibodyautoimmune diseasedigital pathologycomputer-aided immunofluorescence microscopyThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA) allows ...
a) Peripheral or homogeneous pattern on routine IFA b) Definitive detection and quantitation is performed on more specific substrates 1. IFA using the hemoflagellate Crithidia luciliae as substrate 2. Very specific for SLE 3. 40-60% of patients with SLE have anti- ...
However, only 12.3% of them had a titer 鈮 1/320. The sensitivity of the ANA in patients with SLE as assessed by IFA was more than 98%. Nuclear membrane and DFS70 patterns are not specific to any connective tissue disease and they can be detected positive in rheumatoid arthritis and in...
The falsely detected positive samples were all of low titer (1:80). System A found 157 and System B 156 patterns in agreement to the target pattern. From 80 negative samples system A detected 73 and system B 71 samples correctly.ConclusionsBoth systems resulted in an overall sensitivity >95...