speckled pattern; nucleolar pattern; and peripheral or rim pattern. While these patterns are not specific to any one illness, certain illnesses can more frequently be associated with one pattern or another. The patterns can sometimes give the doctor further clues as to the types of illnesses to ...
Peripheral:Fluorescence occurs at the edges of the nucleus in a shaggy appearance. Anti-DNA and anti-nuclear envelope antibodies cause this pattern. Seen in systemic lupus erythematosus. Speckled:Speckled fluorescence due to an antibody directed against different nuclear antigens. This pattern is common...
Indirect immunofluorescence technique applied on HEp-2 cell substrates provides the major screening method to detect ANA patterns in the diagnosis of autoimmune diseases. Currently, the ANA patterns are mostly inspected by experienced physicians to identify abnormal cell patterns. The objective of this ...
?heat damage of slides; this problem can be easily identified by using a Diluent control well that will show nucleolar staining; the Homogeneous Control will also show an abnormal pattern. ?unclear glassware (staining dishes) or poorly deionized water used for PBS preparation; this problem will ...
Homogenous (46%) and speckled (31%) were the most frequently observed staining patterns at inclusion, whereas 7% switched their pattern at least once during follow-up, see figure 1. Established associations between ANA fine-specificities and clinical data were confirmed. Levels of anti-Sm/RNP, ...
An important observation is that, while the Homogeneous (AC-1) and Coarse Speckled nuclear (AC-5) patterns are linked to autoantibodies strongly associated with SARD, the Dense Fine Speckled (DFS) nuclear pattern (AC-2) virtually rules out a SARD diagnosis. A clear cut DFS pattern is ...