traditional bulk RNA-seq only provides the average gene expression per tissue or cell culture, which hides the variability between individual cells and makes it challenging to analyze minor cell subpopulations. To address
Selective BCL-2 inhibition by ABT-199 causes on-target cell death in acute myeloid leukemia. Cancer Discov. 2014;4(3):362–75. Article CAS PubMed Google Scholar Condoluci A, Rossi D. Mechanisms of resistance to venetoclax. Blood. 2022;140(20):2094–6. Article CAS PubMed Google Scholar...
DNA methyltransferase 3 A (DNMT3A) is the most frequently mutated gene in acute myeloid leukemia (AML). Although chemotherapy agents have improved outcomes forDNMT3A-mutant AML patients, there is still no targeted therapy highlighting the need for further study of howDNMT3Amutations affect AML p...
The overexpression of the ecotropic viral integration site-1 gene (EVI1/MECOM) marks the most lethal acute myeloid leukemia (AML) subgroup carrying chromosome 3q26 abnormalities. By taking advantage of the intersectionality of high-throughput cell-based and gene expression screens selective and pan-...
effect on prognosis and treatment. Patients with AML average 5 recurrent gene mutations. Patients with mutations inNPM1, or inCEBPAhave a more favorable prognosis. Mutations inFLT3, on the other hand, have a poorer prognosis (including in patients who also have an otherwise favorableNPM1mu...
(Lei et al.2021). Since single-cell material is low, producing a larger quantity usually needs an amplification step. However, because of the amplification’s nonlinearity, which also causes bias and an unbalanced cDNA ratio in the cells, some markers cannot be amplified (Qi et al.2020). ...
Approximately one third of normal karyotype AML cases (35%) have an abnormality in the C-terminus of the protein that causes it to be present in the cytoplasm of affected cells rather than restricted to the nucleus.29 Although gene array analysis has revealed an up-regulation of genes ...
In the gene mutation map of AML, FMS like receptor tyrosine kinase 3 (FLT3) gene mutation is the most common, which exists in about one third of newly diagnosed patients. FLT3 mutation mainly includes tandem repeat mutation (ITD) in transmembrane region and point mutation (TKD) involving activ...
Many patients with acute myeloid leukemia (AML) share a mutation in a gene called IDH. A University of Colorado Cancer Center study published this week in the journal Leukemia & Lymphoma shows that this IDH mutation may be the first domino in a chain tha
Blood 116: 5089 – 5102 Kiyoi H, Towatari M, Yokota S, Hamaguchi M, Ohno R, Saito H, Naoe T (1998) Internal tandem duplication of the FLT3 gene is a novel modality of elongation mutation which causes constitutive activation of the product. Leukemia 12: 1333 – 1337 Kiyoi H, Yanada ...