Objective To establish kinetic assay method for alanine aminopeptidase(AAP)in serum, be applied to clinical diagnosis primarily. 目的建立血清丙氨酸氨基肽酶(AAP)连续监测法。 3. Objective To establish a kinetic monitoring assay for detecting glycylproline dipeptidyl aminopeptidase (GPDA). Methods Gly-pro...
(CWBIO, Beijing, China). The concentration of protein was determined by BCA assay kit (Biosynthesis Biotechnology, Beijing, China). Purified recombinant protein was confirmed by mass spectrometry (Applied Biosystems, Foster City, CA). 2.4. Western Blotting Human umbilical vein cell line (EA.hy926...
It is possible that an APB-assay in serum could show the efficacy of the treatment with antibiotics in the cases described. The APB-like enzyme studied differed from the rat liver enzyme as regards substrate specificity and molecular weight, but resembled it in several other properties.Kauko...
Define Aminopeptidases. Aminopeptidases synonyms, Aminopeptidases pronunciation, Aminopeptidases translation, English dictionary definition of Aminopeptidases. n. Any of various enzymes that catalyze the hydrolysis of the terminal peptide bond of a polyp
Tsuru, A novel assay method for aminopeptidase P and partial purification of two types of the enzyme in Escherichia coli, Agric. Biol. Chem. 52 (1988) 1957- 1963.Yoshimoto T, Murayama N, Tsuru D. Novel assay method for aminopeptidase P and partial purification of two types of the enzyme ...
(b) S. exigua BBMV proteins binding Cry1Ac in ligand blots, as detected with Cry1Ac antisera. Panel 1, blotting assay without Cry1Ac, Panel 2, blotting assay with Cry1Ac. Arrows indicate detected Cry1Ac-binding protein bands. Banda 1 2 Accession numberb Q4G6A5 Q5UVJ2 PepCount 35 28 ...
(Bockelmannet al., 1992), was inhibited completely by 0.1 mM EDTA, and its activity was increased by 1 mM Mn2+and 0.1 mM Hg2+; suitable substrates for the assay of enzyme activity were L-Lys-Na and L-Ala-L-Arg-NA. A similar enzyme fromL. helveticushad a primary sequence PepN ...
Enzyme activity assay and substrate specificity analysis. Aminopeptidase activity was deter- mined spectrophotometrically in the assay buffer (50 mM Tris-HCl, pH 8.5) containing 2 mM amino acid-p-nitroanilide (XXX-pNA) substrate. Absorbance at 405nm, due to the release of p-...
N-Leucyl-m-aminobenzenesulfonate exhibits desirable properties (solubility much greater than Km,Δϵ at 295 nm of 2000 m−1 cm−1, and V of 300 μmol min−1 mg−1) as a substrate for a spectrophotometric assay of leucine aminopeptidase. With the exception of N-leucyl-p-amino...
Development and Validation of a Whole-Cell Inhibition Assay for Bacterial Methionine Aminopeptidase by Surface-Enhanced Laser Desorption Ionization-Time of... .Development and validation of a whole-cell inhibition assay for bacterial methionine aminopeptidase by surface-enhanced laser desorption ionization-...