Prokaryotic expression, purification, and polyclonal antibody production against a novel drug resistance gene of Leishmania donovani clinical isolate. Protein Expr Purif 45, 15–21, doi: 10.1016/j.pep.2005.10.00
Expression and purification of recombinant proteins and polyclonal antibody production According to the nucleotide sequences of the cad gene (GenBank accession no. AF519180), the apn4 gene (GenBank accession no. AY181026) and the alp2 gene (GenBank accession no. EU729323) of H. armigera, th...
ME1 (IgG1), an anti-HLA-B*07/B*27/B*22 monoclonal antibody (mAb) that recognizes HC/β2m/peptide complexes (37), was used for immunopurification of HLA-B*27. Among the non-B*27 MHC-I molecules expressed in the cell lines used in this study, ME1 is known to have a weak cross...
Allosteric inhibition of BACE1 by an exosite-binding antibody December 2013 Weiru Wang,…, Robert A Lazarus Regulation of deubiquitinase proteolytic activity December 2013 Oscar W Huang, Andrea G Cochran Modulating caspase activity: beyond the active site December 2013 Jeremy Murray, Adam R Renslo Vie...
antibody production. The cloning process is repeated until 100% of the sub-population exhibits the production of a specific antibody, which is indicative of the “stability” of the hybridoma. In addition, hybridoma culture wells often have multiple colonies some of which may be antibody non-...
Bartling D, Nosek J. Molecular and immunological characterization of leucine aminopeptidase inArabidopsis thaliana: A new antibody suggests a semi-constitutive regulation of a phylogenetically old enzyme. Plant Sci 1994; 9: 199–209. Google Scholar ...
A study on Fula ethnic group (the largest ethnic groups in the Sahel and West Africa) showed that the presented antigens by DQA-DQB are associated with the higher levels of antibody production [51]. With regard to the geographical location and ethnic genome polymorphisms, prediction of the ...
II. Production and Recovery of Cleaved Polypeptide In this alternative process, the polypeptide is contacted with the aminopeptidase b2323 polypeptide directly so that it is clipped. This may be accomplished by several means, including incubation therewith at about 20-40° C., preferably about 30...
Moreover, the existence of “common mucosal immunity” indicates that stimulation of the nasal mucosa will result in antibody production in the intestinal mucosa [74]. Upon the stimulation with the KKGIKC-BSA conjugate, cells produced considerable amounts of IL-6. The IL-6 is a cytokine that...
Right here we used AID-deficient mice to supply a distinctive tool to research the function of IgM, as the lack of AID network marketing leads to antibody repertoires limited to IgM in humans and mice [15] exclusively. greater putting on weight in receiver mice weighed against FMT using ...