The dimer configuration of IRAP could permit the transfer of information across the cell membrane similar to the dimer structure of AP-N which mediates an IP3-linked elevation of intracellular calcium in monocy
The first 21 residues form a hydrophobic stretch that may function as a signal sequence for the endoplasmic reticulum, and the remaining 35 residue segment accounts for the 4 kDa difference between the proform and mature aminopeptidase Y. Anti-aminopeptidase Y antibody adsorbs over half the ...
Protein concentration was quantified using the NanoDrop (Thermo Fisher Scientific, Lafayette, US) for the following polyclonal antibody preparation and aminopeptidase activity assay. Preparation of polyclonal antibodies against the recombinant proteins. The purified recom- binant protein was used for ...
Bartling D, Nosek J. Molecular and immunological characterization of leucine aminopeptidase inArabidopsis thaliana: A new antibody suggests a semi-constitutive regulation of a phylogenetically old enzyme. Plant Sci 1994; 9: 199–209. Google Scholar ...
Subsequently, plates were incubated with anti-swine IgM-, IgA-, or IgG- specific monoclonal antibody (28.4.1, 27.8.1, and 23.3.1b, respectively36) in ELISA dilution buffer for 1 h at 37 °C, followed by rabbit anti-mouse horseradish peroxidase conjugate (Dako) optimally diluted in ELISA ...
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A, B Confocal microscopy images. We measured the binding activities of peptides with amino acid mutations (green, FITC labelled) and F4 FaeG mutants in the proximal jejunum of piglets using confocal microscopy. We tested the F4 strains in these cells by using monoclonal antibody against F4 and...
a. True b. False I am a protein that circulate in the blood and binds to a specific antigen. My name is antibody. True False True or False. Substances such as glucose, proteins, and erythrocytes are secreted into the filtrate. Say true or false: Lipids contain less ene...
Other examples of biological diagnostic devices, which can be used for the detection of es-LAPase, using the monoclonal antibody of the present invention, include the devices described by G. Grenner, P. B. Diagnostics Systems, Inc., in U.S. Pat. Nos. 4,906,439 and 4,918,025. ...
After washing, the titer of bound immunoglobulin can be determined using, for example, a goat anti-mouse antibody conjugated to horseradish peroxidase. After additional washing, the amount of immobilized enzyme is determined (for example, through the use of a chromogenic substrate). Such screening ...