The aminopeptidase activity, tested with L-Arg beta-naphthylamide and with Arg0-Met-enkephalin as substrates, was inhibited by o-phenanthroline, and restored by Zn2+ suggesting its metallopeptidase character. The partial characterization of an aminopeptidase-B activity in rat brain cortex identified a ...
Aminopeptidase B, an arginyl aminopeptidase, was purified from goat brain with a purification factor of 280 and a yield of 2.7%. It was entrapped in calcium alginate together with bovine serum albumin. The optimal conditions for immobilization for maximum activity yield were 1% CaCl2 and 2.5% ...
activityby the treatment of cells withpapain, under conditions that do not affectcell viability. Theaminopeptidaseactivity of individual cells in cytocentrifuge preparations can also be measured as described by Wachsmuth and Stoye using leucine-4-methoxy-2-napthylamide substrate andfast blueB salt. ...
Aminopeptidase activity of Ureaplasma urealyticum. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec; 82 (6):917–918. [ PubMed ]Vinther O, Black FT. Aminopeptidase activity of Ureaplasma urealyticum. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec; 82 (6):917–918....
Synonyms Inflammation ; Leukotriene B 4 ; Lipid mediator Definition Leukotriene A 4 hydrolase/aminopeptidase (LTA4h) (EC 3.3.2.6) is a bifunctional zinc metalloenzyme with both an epoxide hydrolase and aminopeptidase activity (Fig.聽 1 ). Its primary and most well-recognized function is to ...
The cell free extracts of these strains were all found to possess LBS hydrolytic activity as well (Fig. 2). However, the boiled CFE of E. coli DH10B cannot perform this process, demonstrating that LBS hydrolysis is not a spontaneous process and that there is a conserved hydrolase gene ...
The aminopeptidase activity levels and the total protein concentrations of the hemocytes and sera of these infected snails were determined. It was ascertained that snails parasitized by larvae of L. setiferoides had significantly higher levels of hemocytic aminopeptidase activity and hemocytic total ...
<div p-id="p-0001">The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also p
coli genome sequencing project (Blattner et al., Science, 277: 1453-62 (1997)) in the GenBank database (accession number AE000321), but no further information on its enzyme activity is provided. The homolog in E. coli strain O157:H7 is identical to the yfcK gene in the K12 strain. ...
Yuan W, Zhong Z, Wong C-H, Haeggstro¨m JZ, Wetterholm A, and Samuelsson B (1991) Probing the inhibition of leukotriene A4 hydrolase based on its aminopep- tidase activity. Bioorg Med Chem Lett 1:551-556.Yuan, W., Zhong, Z., Wong, C.H., Haeggström, J.Z., Wetterholm, ...