magnaporthe oryzaepathogenicityproteomericetranscriptomeAlternative splicing (AS) contributes to diversifying and regulating cellular responses to environmental conditions and developmental cues by differentiall
Analyses of alternative splicing events revealed that the number of all alternative splicing events (10034), intron retentions (IR, 9369), alternative 5’ splice sites (A5SS, 167), and alternative 3’ splice sites (A3SS, 302) is 7.3, 7.4, 5.1, and 5.9-fold higher, respectively, than ...
originally introgressed from tetraploidTriticum carthlicum22. Constitutive alternative splicing (AS) ofPm4generates two isoforms, both required for resistance, with different domain architectures forming an endoplasmic reticulum (ER)-associated complex revealing an additional and unique molecular basis for a ...
giving rise to the phenomena of splice variation and alternative splicing. In this paper we use 'splice variation' to describe any difference in intron processing, reserving the term 'alternative splicing' for splice variation that is regulated and functionally significant. ...
FPA contains a RRM but the precise mechanism by which FPA controls 39 end formation (and possibly other processes such as splicing) of RNA targets is unclear. FPA controls polyadenylation of its own transcript and of others by promoting polyadenylation at the proximal site, thereby inhibiting ...
While both PacBio and Nanopore have smaller through-put, they are excellent for obtaining long reads and other information like alternative splicing (PacBio) or RNA modifications (Nanopore). Ribo-seq can be used to confirm the efficacy of the outcome of APA by exam- ine if the transcripts ...
The RT-qPCR technique was basically performed as previously described, with slight variations35. Briefly, the total RNA extracted from 106trophozoites/mL ofP. dicentrarchicultured under hypoxic/normoxic conditions, in the presence of 1 mM SHAM and 1 mM KCN (inhibitors of AOX and cytochrome-complex...