[應用範例 Biomarker/cytokine detection] Protocol optimization for detection and quantification of membrane-bound proteins using AlphaLISA technology [應用範例 Biomarker/cytokine detection] Quantifying TNFR1 in both soluble and membrane bound form using AlphaLISA technology [應用範例 Biomarker/cytokine detection...
Representative Data Data obtained with a 2-plate, 2-incubation protocol. HeLa cells were plated at 40K cells per well in a 96 well plate and incubated overnight. Cells were then treated with IL-4 at indicated concentrations for 20 minutes. Cells were lysed in 1X Lysis Buffer and assayed ...
Product Information (2)Quality Control ...2 Analyte of Interest .. (3)Description of the AlphaLISA Assay ...3 Precautions . (3)Kit content: Reagents and Materials..
The customized donor bead enables sensitive, high-throughput immunoassay screening of photosynthetic tissues within the plant biotechnology industry using a convenient, photostable protocol.Aaron J. WalckKristi R. HarkinsJALA: Journal of the Association for Laboratory Automation...
Once we determined the initial conditions, we adapted the protocol for differentially tagged TUBEs reagents. For the acceptor bead, there are several capture modalities to choose from depending on the E3 and its modifications. The Mdm2 full length E3 ligase used in this assay is His-tagged. The...
Data obtained with a 2-plate, 2-incubation protocol. Jurkat cells were harvested, washed and resuspended in HBSS + 0.1% BSA at a density of 4 x 106 cells/mL. Cells were then treated with pervanadate at the indicated concentrations for 30 minutes. Cells were lysed with 5x Lysis Buffer and...
AlphaLISA assay performance described below was determined using the standard protocol. Sensitivity: The LDL was calculated as described above. The values correspond to the lowest concentration of analyte that can be detected in a volume of 20 µL using the recommended assay conditions. ...
coli; Shiga toxin; STEC; Stx2 Key Contribution: There are many benefits to the newly developed AlphaLISA including a shorter and simpler homogeneous protocol that requires less manual manipulation and no wash steps compared to the ELISA. Overall, this AlphaLISA allows for the rapid detection of ...