In-Cell ELISA ProtocolRequired, Materials
In-Cell ELISA is a quantitative immunocytochemistry method to measure protein levels or post-translational modifications of cultured adherent cells. The cells are grown in and fixed to the bottom of a coated 96-well plate. Targets of interest are detected by primary antibodies, which are in turn ...
UsingIn-Cell ELISAover Western Blot provides a more accurate and quantitative assay for detecting proteins in cells. The ease of performance and shorter protocol makes it a more convenient assay. In-Cell ELISA is becoming the assay of choice for protein analysis and should be considered when ...
Version 1 Last updated 26 June 2017 ab207491 In-Cell ELISA Support Pack for Suspension Cells (Chemiluminescent)For use with In-Cell ELISA kits to facilitate washing and liquid handling steps for suspension cells.This product is for research use only and is not intended for diagnostic use.Copyrigh...
Monitoring T cell proliferation with CellTrace Violet Cell Proliferation Kit Measuring cell distribution in S-phase with BrdU staining Measuring cell distribution in S-phase with Click-IT Edu staining Compensation beads protocol Validated antibody panels, OMIP (optimized multicolor immunofluorescence panels)...
ELISA Sample Preparation Protocols Protocols 96-Well Sample Preparation for Suspension Cells 96-Well Sample Preparation for Adherent Cells Cell Extraction Protocol Nuclear Extraction Protocol Plasma and Serum Preparation Rat Blood Collection Protocols
1c). This yielded seven major cell types, comprised of various types of immune cells: T-cells, NK-cells, B-cells, myeloid cells, and plasmacytoid dendritic cells, followed by erythroid cells and stem cells, which were supported by expression of canonical marker genes (Fig. 1d)43,44,45,...
Human IgG1Human IgG3Mouse IgG1Mouse IgG2aGoat Polyclonal EGFR Lymphoma cell GD2 CD4 Apolipoprotein-A2 HER2/neu GD3 β-tubulin CD3 sLea sLea CD8a CD8 GD2 f-GM1 CD45 CD56 GM2 Tn C3b, iC3b C3b, iC3b, C3dg J591 Interferon-γ SiteClick Antibody Labeling Kits selection guide Product name...
3.Single cell atlas identifies lipid-processingand immunomodulatory endothelial cellsin healthy and malignant breast 4.An Optimized Flow Cytometry Protocol for Analysis of Angiogenic Monocytes and Endothelial Progenitor Cells in Peripheral Blood
Cell-free genetically encoded biosensors have been developed to detect small molecules and nucleic acids, but they have yet to be reliably engineered to detect proteins. Here we develop an automated platform to convert protein-binding RNA aptamers into r