(aa 35‑211) that shows one WIF-1-like domain (aa 51‑180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318‑581). There is one alternate start site at Met117. Over aa 35‑211, mouse Ryk is 94% aa identical to human Ryk, and ...
Recently, aqueous Wnt signaling modulators, Wnt inhibitory factor 1 (WIF-1) and Dickkopf 3 (DKK-3) are reported significantly higher in neovascular AMD patients, the high WIF-1 levels are also associated with disrupted length of photoreceptor inner and outer segments [56]. This finding supports...
between DKK1 and LRP6 was determined in an ELISA-based binding assay utilizing purified DKK1 protein and purified LRP6 ectodomain-Fc fusion protein (R&D Systems). Based on their ability to inhibit DKK1 functionin vitroand their binding mapping to distinct domains of DKK1, two FAbs were selected f...
between DKK1 and LRP6 was determined in an ELISA-based binding assay utilizing purified DKK1 protein and purified LRP6 ectodomain-Fc fusion protein (R&D Systems). Based on their ability to inhibit DKK1 functionin vitroand their binding mapping to distinct domains of DKK1, two FAbs were selected f...
Like SFRPs, WNT inhibitory factor 1 antagonizes Wnt sig- naling by binding to WNTs. Wif1 KO mice have a normal skeleton but are sensitive to radiation-induced osteosar- comas163 and mice with osteoblast-specific Wif1 overex- pression display no overt bone phenotype.164 Lexicon's Wif1 KO...
temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog #NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol forFluorescent ICC Staining of Cells on...