Like SFRPs, WNT inhibitory factor 1 antagonizes Wnt sig- naling by binding to WNTs. Wif1 KO mice have a normal skeleton but are sensitive to radiation-induced osteosar- comas163 and mice with osteoblast-specific Wif1 overex- pression display no overt bone phenotype.164 Lexicon's Wif1 KO...
between DKK1 and LRP6 was determined in an ELISA-based binding assay utilizing purified DKK1 protein and purified LRP6 ectodomain-Fc fusion protein (R&D Systems). Based on their ability to inhibit DKK1 functionin vitroand their binding mapping to distinct domains of DKK1, two FAbs were selected f...
(aa 35‑211) that shows one WIF-1-like domain (aa 51‑180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318‑581). There is one alternate start site at Met117. Over aa 35‑211, mouse Ryk is 94% aa identical to human Ryk, and ...