Alpha-synuclein preformed fibrils:a tool to understand Parkinson's disease and develop disease modifying therapy degenerationAlphadrivingParkinson's disease(PD)is the second most common neurodegenerative disorder characterized by multiple motor and nonmotor symptoms,which include,among others,constipation,sleep...
To test this hypothesis, we used preformed α-Syn fibrils (PFF) and stereotactically injected them into the right striatum of Sprague-Dawley rats. This method accurately simulated the pathological characteristics of diffuse pS129 in the brains of PD-Dep patients. Two months after the injection, b...
We studied the microglial uptake of alpha‐synuclein preformed fibrils (PFFs) and the effect of pro‐inflammatory stimulation by interferon gamma. We demonstrate that combined exposure disrupts the phagosome maturation pathway while inflammatory stimuli suppress chaperone mediated autophagy and mitochondrial ...
P- and S129A-PFF. Equal amounts of fibrils were analyzed in a 10% SDS-PAGE gel using the C20 antibody. P-PFF were detected with the α-Synuclein (phospho Ser) antibody whereas no signal was observed for the S129A- and wt-PFF. (b) Fibril formation monitoring by Th-S assay. Graphs...
A-syn pathology can be observed in in vitro and in vivo models through the introduction of pathological α-syn seeds in the form of preformed fibrils (PFFs) from recombinant α-syn monomers [13], α-syn obtained from brain samples of patients [14] or rodents carrying α-syn mutations [15...
Alpha-Synuclein Preformed fibrils Synucleinopathy Parkinson's disease Sorry, something went wrong. Please try again and make sure cookies are enabledCited by (66) Animal models of brain-first and body-first Parkinson's disease 2022, Neurobiology of Disease Show abstract Neuropathological evidence of...
(αsynO-β) are toxic to cells, possibly through physical disruption of cellular membranes.2,3αSynO-β/preformed fibrils (PFFs) generated in vitro or isolated from the brains of patients developing synucleinopathies can “seed” aggregates, especially in transgenic rodent models overexpressing α...
Indeed, PD pathology can be induced in the brain and spinal cord of α-Syn transgenic mice expressing mutant A53T human α-Syn (M83 line) by a single periph- eral intramuscular injection of α-Syn preformed fibrils (PFF) [47]. This increased face-value of the M83 prion- like model ...
activities might be a driving mechanism that can determine the pathogenesis of different synucleinopathies. More recently, fibrils prepared with homogenous N- or C-terminally truncated αSyn were shown to exhibit distinct prion-like cross-seeding activities in mice50. Together with our findings, this...
A-syn pathology can be observed in in vitro and in vivo models through the introduction of pathological α-syn seeds in the form of preformed fibrils (PFFs) from recombinant α-syn monomers [13], α-syn obtained from brain samples of patients [14] or rodents carrying α-syn mutations [15...