Allele-specific PCR in SNP genotyping. In: Single Nucleotide Polymorphisms. Humana Press, 2009. 415-424.Gaudet, M., A. Fara, I. Beritognolo, and M. Sabatti. 2009 . Allele-specific PCR in SNP genotyping . Methods in Molecular Biology 578: 415 – 424 . ,...
Compact zinc finger architecture utilizing toxin-derived cytidine deaminases for highly efficient base editing in human cells Friedrich Fauser Bhakti N. Kadam Jeffrey C. Miller Nature Communications(2024) Development of super-specific epigenome editing by targeted allele-specific DNA methylation ...
PACE™ (PCR Allele Competitive Extension) genotyping is a fluorescent, competitive allele-specific PCR genotyping technology. It is ideal for biallelic discrimination of single nucleotide polymorphisms (SNPs) and insertions and deletions (indels) at specific loci. ...
Genotyping-by-sequencing (GBS) based single nucleotide polymorphisms (SNP) enabled Kompetitive allele specific PCR (KASP) marker development in mutant Rubus genotypesdoi:10.1016/j.ejbt.2018.08.001Ryu, JaihyunkKim, Woon JiIm, JuhyunKim, Sang Hun...
restriction enzyme digestion. In addition, for high-throughput genotyping using a fluorescence detection system, we also developed Fluidigm SNP genotyping markers. Moreover, the allele-specific PCR-gel based markers were validated through its application in our marker-assisted selection (MAS) breeding ...
BMC Genomics (2015) 16:908 DOI 10.1186/s12864-015-2180-2 RESEARCH ARTICLE Open Access Comparison of Kompetitive Allele Specific PCR (KASP) and genotyping by sequencing (GBS) for quality control analysis in maize Berhanu Tadesse Ertiro1*, Veronica Ogugo2, Mosisa Worku2, Biswanath Das2, ...
For a wide range of diseases, SNPs in the genome are the underlying mechanism of dysfunction. Therefore, targeted detection of these variations is of high importance for early diagnosis and (familial) screenings. While allele-specific PCR has been around
In this work, we focus on a simple and cost-effective protocol, namely the allele-specific polymerase chain reaction or AS-PCR genotyping technique. This assay protocol were adopted by many recent studies, for instance, the detection of common alleles of cytochrome P450 that mediates different ...
p pBackground/p pAllele-specific (AS) Polymerase Chain Reaction is a convenient and inexpensive method for genotyping Single Nucleotide Polymorphisms (SNPs) and mutations. It is applied in many recent studies including population genetics, molecular genetics and pharmacogenomics. Using known AS primer ...
Long-Range Allele-Specific PCR Three independent long-range PCRs are performed per sample: one reaction amplifies both R117H alleles of the heterozygous R117H sample, while the other two are allele-specific (Figure 1). Primer sequences are shown in Table 2. The three PCRs use a common re...