位点特异性PCR 1. In addition,a pair ofallele-specific PCR(AS-PCR) primers were designed to authenticate C. 另外,还设计出了鉴别番红花的位点特异性PCR引物,无需测序即可对番红花及其混淆品进行准确的分子鉴别。 更多例句>> 4) specific PCR
allelepcrspecificniculescumixagarose MihaiNiculescuPage13/1/2007Allele-specificPCR(AS-PCR)(forthehumanPEMTsnprs12325817)PrincipleofthemethodSelectivePCRamplificationofoneoftheallelestodetectSingleNucleotidePolymorphism(SNP).Selectiveamplificationisachievedbydesigningaprimersuchthattheprimerwillmatch/mismatchoneoftheallel...
We used specific antibodies for each isoform (recognizing the distinct C termini) (Cassago et al., 2012) and/or a common antibody, recognizing the N terminus shared by the two isoforms, depending on the cell line. Although the two isoforms share the same active site, GAC has a higher ...
2) Allele-specific amplification 等位基因特异扩增法 1. Objective:To establish Allele-specific amplification(ASA-PCR)for study the distribution of MDR1 polymorphism in transplant patients. 目的:建立等位基因特异扩增法(Allele-Specific Amplification,ASA-PCR)研究移植患者多药耐药基因(multi-drug re- si...
F.; 1999: Nested allele-specific PCR primers distinguish genetic groups of Uncinula necator. Appl. Environ. Microbiol. 65, 3950-3954.Delye C, Ronchi V, Laigret F and Corio-Costet MF, Nested allele-specific PCR primers distinguish genetic groups of Uncinula necator. Appl Envir Microbiol 65:...
The invention relates to an AS-PCR (allele-specific polymerase chain reaction) primer design method, a gene polymorphism detection method and a kit. The method and the kit are used for detecting a target sequence possibly containing an allelic variation area to determine whether an allelic ...
五引物单管聚合酶链反应(pcr)扩增法快速测定人cyp2d610等位基因的类型 an efficient approach to human cyp2d610 allele-specific typing by polymerase chain reaction with five primers in a single tube.pdf 2015-01-29上传 五引物单管聚合酶链反应(pcr)扩增法快速测定人cyp2d610等位基因的类型 an efficient ...
The length of each allele is determined by PCR using specific oligonucleotides primers flanking the repeated sequence.5个回答 通过使用特定的寡核苷酸引物,侧翼重复序列PCR确定每个等位基因的长度。2013-05-23 12:21:38 回答:匿名 每个等位基因的长度是确定的或寡核酸pcr使用特定的底漆穿插重复序列。 2013-05...
Using known AS primer design tools to create primers leads to cumbersome process to inexperience users since information about SNP/mutation must be acquired from public databases prior to the design. Furthermore, most of these tools do not offer the mismatch enhancement to designed primers. The ...
The polymorphism SNP c.987G > C (rs12895357) was determined in an allele-specific manner using two different reverse primers that differ in the base position of SNP c.987 and were each labeled with a different fluorophore, WT sequence G with IRD700 dye and SNP sequence C with Cy5 fluorop...