The different alleles of a given gene determine the degree to which the specific hereditary characteristic controlled by that gene is manifested. The particular allele which causes that characteristic to be expressed in a normal fashion is often referred to as the wild-type allele. Mutations of ...
This kind of outputs should help users tailor their final primers to fit the AS-PCR assay. Conclusion We presented WASP, a web-based allele specific primer design application. The tool conveniently assists scientists in getting SNP information from local SNP database, mirrored from all major ...
a step of performing PCR of the genomic DNA using the primer set; and a step of analyzing the PCR product and determining the coat color and sex.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Multiplex Allele Specific PCR mimetic diagram; (BB) M-ASP; (CC) Internal control group : 308bp...
Development of super-specific epigenome editing by targeted allele-specific DNA methylation Nivethika Rajaram Alexandra G. Kouroukli Albert Jeltsch Epigenetics & Chromatin(2023) hESC-derived striatal progenitors grafted into a Huntington’s disease rat model support long-term functional motor recovery by...
specific epigenetic map with unprecedented resolution in gynogenetic and androgenetic embryos, respectively, and identify 1,279 allele-specific CHMs, including 19 known imprinting control regions (ICRs). Our study suggests that 22 ICR-like regions (ICRLRs) may regulate allele-specific transcription ...
specific genomic coverage files and generates a tab-delimited table for the user-defined regions of interest. Mouse and human exon, gene body and transcription start site coordinates are provided to facilitate analyses of such regions.bVenn diagram showing the theoretical number of CpG dinucleotides ...
The present invention is an improvement of the selective amplification of nucleic acids, which uses allele-specific suppression of amplification of the undesired variants of the target sequence. A schematic diagram of the method of the present invention is shown on FIG. 1. The diagram shows a dou...
Based on the transcriptome-wide analysis of allele-specific expression using RNA sequencing technology, 305 allele-specific imprinting genes (ASIGs) were identified in embryos, and 655 ASIGs were identified in endosperms from three maize F1 hybrids. Of these ASIGs, most did not show consistent ...
This difference in phenotypes likely reflects the differential expression patterns of the two genes; while AXIN1 is ubiquitous in various tissues [9], AXIN2 shows a more restricted developmental and cell-type–specific expression pattern [4]. Additionally, AXIN2, but not AXIN1, is a major ...
Parent-of-origin-specific expression of imprinted genes is critical for successful mammalian growth and development. Insulin, coded by the INS gene, is an important growth factor expressed from the paternal allele in the yolk sac placenta of therian mamm