A new water-soluble porphyrin having the phosphate group, 5,10,15,20-tetrakis(4-phosphonooxyphenyl)porphine, was used as an ultra highly-sensitive chromogenic reagent of alkaline phosphatase enzyme activity. In the spectrophotometric determination of trace amounts of alkaline phosphatase with this ...
The present invention includes the construction of various mutations in the gene Alkaline Phosphatase of Escherichia coli resulting in the production of an enzyme having a specific activity improved. MUTANT ENZYMES ACTIVITY RESULTING ELEVATE ABOVE SPECIFIED 1.5 to 36 times, while maintaining a temperature...
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hy...
~3,000 U/mg enzyme protein (+37℃, 4-nitrophenyl phosphate substrate, diethanolamine as buffer) packaging【包装】 pkg of 1.5 mL (15 mg) manufacturer/tradename Roche optimum pH【最佳pH】 9.8 pH range【pH范围】 9.0 - 11.0 shipped in【运输】 ...
WellVolumeReagent AS1 - AS750 µLSerial Dilution (0.1 to 100 mU/mL) BL50 µLH2O - 0.1% BSA TS50 µLtest sample In supernatants: Prepare alkaline phosphate standards (AS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384...
Phosphate-containing buffers should be avoided when performing alkaline phosphatase enzyme assays. Converting your protocol General overview of optimization steps There are many factors that contribute to the performance of an ELISA. These include capture antibody concentration, detection antibody concentration...
1. Schematic diagram of the 2 alkaline phosphatase (AP) assays using (a) in- soluble substrates (Enzyme- Labeled Fluorescence, ELF) and (b) soluble substrates (MFP, FDP, AttophosTN)). MFP = 3-0-methylfluorescein phosphate. FDP = 3,6-fluo- rescein diphosphate, CPPCQ = 2-(5'-chloro...
It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions. General Description Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. Biochem/physiol Actions Alkaline phosphatase is a model enzyme for ...
mutant K328H has an activity which is comparable to that of the wild-type enzyme; by contrast, at pH 8 and in the absence of a phosphate acceptor, this mutant exhibits a significant decrease in catalytic activity, as compared with that of the wild-type enzyme. These results suggest that ...
A method is described for the highly sensitive chemiluminescence (CL) assay for alkaline phosphatase (ALP) using cortisol-21-phosphate (cortisol-21-P) as substrate and lucigenin as CL reagent; it is applied to an enzyme immunoassay (EIA) for human chorionic gonadotropin (hCG), human growth hor...