M. (1988). Alkaline phosphatase is an ec- toenzyme that acts on micromolar concentrations of natural substrates at physi- ologic pH in human osteosarcoma. Arch Biochem Biophys 264: 400-409.Fedde,KN,Lane,CC,Whyte,MP.Alkaline phosphatase is an ectoenzyme that acts on micromolar concentrations of...
Alkaline phosphatase (ALP) was examined in cultured human osteosarcoma cells (SAOS-2) with respect to isoenzyme form, kinetic properties toward two natural substrates, and topography and nature of attachment to the plasma membrane. ALP in SAOS-2 homogenates is the tissue-nonspecific (TNS) isoenzyme...
This is consistent with rate-limiting diffusional encounter of the substrate with active enzyme and indicates that alkaline phosphatase is a "perfect enzyme". However, the reported second-order rate constants of kcat/Km = 6.6 x 10(6) to 4.6 x 10(7) M-1 s-1 are smaller than the ...
Hypophosphatasia (HPP) is due to deficient activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNAP). This enzyme cleaves extracellular s... E Mornet - Sub-cellular biochemistry 被引量: 0发表: 2015年 Clinical Aspects of Hypophosphatasia: An Update rare inborn error of bone and ...
Alkaline phosphatase, or basic phosphatase, is a homodimeric protein enzyme of 86 kilodaltons. Each monomer contains five cysteine residues, two zinc atoms
The phosphatase which was extracted by nonionic detergent was subjected to column chromatography and preparative electrophoresis. Purified phosphatase showed the highest activity at pH 8.8, indicating that this enzyme belongs to alkaline phosphatase. The apparent molecular mass from sodium dodecyl sulfate-...
ALT is thus known as a leakage enzyme. It is often measured in conjunction with aspartate transaminase (AST), alkaline phosphatase, and bilirubin to evaluate liver disease. Some common causes of elevated alanine aminotransferase include liver diseases such as cirrhosis, viral hepatitis, liver tumors,...
Alanine aminotransferase is an enzyme that's found mainly in the liver. The main purpose of alanine aminotransferase is to...
An alkaline phosphatase-conjugated antibody was used to detect the DIG-labeled probes (Roche, 11093274910). TUNEL staining Fragmented DNA in apoptotic cells was detected using the Promega DeadEnd Colorimetric TUNEL System (G7360) according to the manufacturer....
Alkaline phosphatase (AP), well established as a crucial enzyme for bone formation and bone cell differentiation, was also studied. MMP zymography studies were carried out on fresh cusp retrievals obtained at the time of surgery for calcific aortic stenosis. We also studied TN-C effects on MMP-...