M. (1988). Alkaline phosphatase is an ec- toenzyme that acts on micromolar concentrations of natural substrates at physi- ologic pH in human osteosarcoma. Arch Biochem Biophys 264: 400-409.Fedde,KN,Lane,CC,Whyte,MP.Alkaline phosphatase is an ectoenzyme that acts on micromolar concentrations of...
This is consistent with rate-limiting diffusional encounter of the substrate with active enzyme and indicates that alkaline phosphatase is a "perfect enzyme". However, the reported second-order rate constants of kcat/Km = 6.6 x 10(6) to 4.6 x 10(7) M-1 s-1 are smaller than the ...
Alkaline phosphatase (ALP) was examined in cultured human osteosarcoma cells (SAOS-2) with respect to isoenzyme form, kinetic properties toward two natural substrates, and topography and nature of attachment to the plasma membrane. ALP in SAOS-2 homogenates is the tissue-nonspecific (TNS) isoenzyme...
Hypophosphatasia (HPP) is due to deficient activity of the tissue-nonspecific isoenzyme of alkaline phosphatase (TNAP). This enzyme cleaves extracellular s... E Mornet - Sub-cellular biochemistry 被引量: 0发表: 2015年 Clinical Aspects of Hypophosphatasia: An Update rare inborn error of bone and ...
Alkaline phosphatase, or basic phosphatase, is a homodimeric protein enzyme of 86 kilodaltons. Each monomer contains five cysteine residues, two zinc atoms
When liver cells are injured, ALT effectively “leaks” out of those cells, causing it to appear in higher concentrations in the blood panels. ALT is thus known as a leakage enzyme. It is often measured in conjunction with aspartate transaminase (AST), alkaline phosphatase, and bilirubin to ...
Alanine aminotransferase is an enzyme that's found mainly in the liver. The main purpose of alanine aminotransferase is to...
The antibodies bind unique epitopes of the same cell surface-expressed alkaline phosphatase (ALP) on OS cells. ALP is an enzyme family composed of 4 different members19 and the detection of serum ALP activity is a marker for different diseases including OS. As TP antibody binding signal did ...
alkaline phosphatase. We observed that the three MEFs exhibited different reprogramming efficiencies: MEF 1 > MEF 2 > MEF 3 (Extended Data Fig.7a). This differential efficiency of reprogramming was also reflected in the transcriptomes of these cells, as evaluated by the enrichment of a ...
Serum TNAP consists of approximately equal amounts of the bone and liver isoforms of the enzyme. These isoforms have the same protein sequence but differ in O-linked glycosylation (22). Tissue-nonspecific alkaline phosphatase is a zinc metalloenzyme that hydrolyzes a broad range of phosphate mono...