Determination of Michaelis–Menten Enzyme Kinetics Parameters of Alkaline Phosphatase in Clinical SamplesEnzymes are specific biocatalysts which enhance the rate of biological reactions in mild conditions of temperature, pressure and a particular pH and thus have an important role in almost every cellular ...
The second-order rate constant, kcat/km, for catalysis of the hydrolysis of 4-nitrophenyl phosphate by alkaline phosphatase decreases with increasing viscosity in the presence of sucrose or arabinose, with a slope of delta[kcat/Km)0/(kcat/Km)]/delta(eta/eta 0) = 1.4 at pH 8.0, 25 degree...
The objective of the present study was to find out how adsorption on palygorskite and sepiolite minerals modifies the catalytic activity and the Michaelis鈥揗enten kinetics of alkaline phosphatase (ALP). Inhibition kinetics of adsorbed ALP by Cd was also compared to that of the free enzyme. The ...
Soil alkaline phosphatase (ALP) activity was regarded as the endpoint to explore the hormetic responses under different doses of toluene (0.0, 0.1, 0.5, 1.0, 2.0, 3.0, 5.0, 10.0, 50.0 and 100.0μLg 1 ). Subsequently, we conducted the experiments of enzymatic reaction kinetics and pure ...
Introduction to Enzyme KineticsThe purpose of this experiment is to measure the rates of reaction of the enzyme Alkaline Phosphatase with the substrate p-nitrophenol phosphate under varying conditions. The concentration of both substrate and enzyme were diluted and the inhibitor vanadate was utilized to...
Some enzymes are of interest for their beneficial activity (e.g., lactoperoxidase), some for use as indices of processing (e.g., alkaline phosphatase) and some for effects on the quality of dairy products (e.g., plasmin, lipoprotein lipase), which may be either positive or negative for ...
These interactions are relatively weak and may be disrupted readily by high temperatures, acid or alkaline conditions, or changes in the polarity of the medium. Such changes lead to an unfolding of the peptide chains (denaturation) and a concomitant loss of enzymatic activity, solubility, and ...
reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic ...
生物化学 英文Section C Enzyme
Define extracellular enzyme. extracellular enzyme synonyms, extracellular enzyme pronunciation, extracellular enzyme translation, English dictionary definition of extracellular enzyme. n. An enzyme, such as a digestive enzyme, that functions outside the