Improved alkaline comet assay protocol for adherent HaCaT keratinocytes to study UVA induced DNA damage. Mutation Research 15; 122-128, 2007. :Wischermann, K., Boukamp, P., and Schmezer, P. 2007. Improved alkaline comet assay pro- tocol for adherent HaCaT keratinocytes to study UVA-induced ...
However, despite the considerable data published on the in vivo Comet assay and the general agreement within the international scientific community over many protocol-related issues, it was felt that a document giving detailed practical guidance on the protocol required for regulatory a...
15.3K Views. US FDA/National Center for Toxicological Research. The overall goal of the in vivo alkaline comet assay and the enzyme-modified alkaline comet assay, is to concurrently measure direct and oxidative DNA damage at the single-cell level. This
While the results of the present study do not strictly meet our requirement for a positive dose relationship as part of a positive call in the assay, we believe Conclusions The in vivo comet assay performed carefully and according to a generally accepted protocol is capable of detecting DNA ...
In plants, an increasing interest for the comet assay was shown in the last decade. This versatile technique appears to be promising to detect the genotoxic effect of pollutants and to monitor the environment. However, the lack of a standardised protocol and the low throughput of the assay limi...
This Test Guideline includes the recommended use and limitations of the comet assay, and is based on the final protocol (12) used in the validation trial, and on additional relevant published and unpublished (laboratories proprietary) data. 4. Definitions of key terms are set out in Annex 1....
Performed as a single-cell electrophoresis, this assay is especially simplistic, and the results are easily reproducible. DNA breakage can be quantitatively assessed by the induced comet tail regions, which can be measured using a variety of comet software. This protocol will finish within ...
The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]). For complete details on the use and execution of this protocol, please refer to Martin et al. (2021)....
Taken together this modified comet assay protocol allows the facilitated detection of DNA repair in fresh or cryopreserved human PBMC samples with a good sensitivity and reliability and could be useful in human studies addressing the antioxidant status and repair capacity of PBMC....
Despite its widespread application, variations between studies remain problematic, often due to the lack of a common protocol and appropriate test controls. Using positive controls is essential to assess inter-experimental variability and ensure reliable results. Hydrogen peroxide (H2O2) is the most ...